Molecular cytogenetic characterization of the native forage grass trichloris crinite
| Autor: | Pablo Federico Cavagnaro, Patricia Elda Novo, María Laura Las Peñas, Perla Carolina Kozub |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Agricultura Trichloris crinita KARYOTYPE POLYPLOIDY Forage FLUORESCENT IN SITU HYBRIDIZATION (FISH) 04 agricultural and veterinary sciences Biology biology.organism_classification 01 natural sciences TRICHLORIS CRINITA CIENCIAS AGRÍCOLAS Botany 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Agricultura Silvicultura y Pesca Agronomy and Crop Science 010606 plant biology & botany |
| Popis: | Trichloris crinita is a perennial forage grass native to ?and of wide distribution in- arid and semi-arid regions of the American continent. Its forage value in these dry lands obeys, in addition to its good forage quality, to its high tolerance to drought, salinity, trampling and grazing. Genetic and cytogenetic information for T. crinita is very scarce, hindering progress in genetic research and breeding of the species. Chromosome numbers, karyotypes, heterochromatin distribution, physical mapping of rDNA genes (18-5.8-26S and 5S) by FISH, and chromosome pairing in meiotic cells were described, for the first time, in T. crinita accessions. Karyotype based on chromosome morphology and cytogenetic landmarks are presented for eight T. crinita accessions. Physical mapping of rDNA loci revealed variation in the number, size and intensity of the 18-5.8-26S FISH signals, whereas 5S rDNA produced two signals per accession. Considering a basic chromosome number of 10, as reported for most Chloridoideae species, the facts that all 20 T. crinita accessions analyzed had 40 somatic chromosomes, that only bivalent chromosome pairings were observed in meiosis, and that chromosome morphology and sizes revealed high similarity between pairs of chromosomes, along with other reproductive features of the species, strongly suggest that T. crinita is an allotetraploid (2n=4x=40). Our cytogenetic data represent a useful resource for advancing cytogenetic research and breeding in T. crinita. Fil: Kozub, Perla Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Las Peñas, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Novo, Patricia Elda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina Fil: Cavagnaro, Pablo Federico. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-san Juan. Estación Experimental Agropecuaria la Consulta. Agencia de Extensión Rural la Consulta; Argentina |
| Databáze: | OpenAIRE |
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