Brisk production of nitric oxide and associated formation ofS-nitrosothiols in early hemorrhage
| Autor: | Motilal B. Pamnani, Billy W. Day, Nikolai V. Gorbunov, James L. Atkins, Michael T. Handrigan, Zhe Zhang |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
medicine.medical_specialty Erythrocytes Time Factors Physiology Blood Pressure Hemorrhage Nitric Oxide Rats sprague dawley Nitric oxide Rats Sprague-Dawley chemistry.chemical_compound Physiology (medical) Internal medicine NG-Nitroarginine Methyl Ester No synthase medicine Animals Enzyme Inhibitors S-Nitrosothiols Glycated Hemoglobin biology Ceruloplasmin Rats Nitric oxide synthase Endocrinology chemistry Regional Blood Flow biology.protein Nitric Oxide Synthase |
| Zdroj: | Journal of Applied Physiology. 100:1267-1277 |
| ISSN: | 1522-1601 8750-7587 |
| DOI: | 10.1152/japplphysiol.01059.2005 |
| Popis: | The results of previous inhibitor studies suggest that there is some increase in nitric oxide (NO) production from constitutive NO synthase in early hemorrhage (H), but the magnitude of NO production early after H has not been previously assessed. It is generally believed that only modest production rates are possible from the constitutively expressed NO synthases. To study this, anesthetized male Sprague-Dawley rats were subjected to 90 min of isobaric (40 mmHg) H. During this period of time, the dynamics of accumulation of NO intermediates in the arterial blood was assessed using electron paramagnetic resonance spectroscopy, chemiluminescence, fluorescence imaging, and mass spectrometry. Electron paramagnetic resonance-detectable NO adducts were also measured with spin traps in blood plasma and red blood cells. H led to an increase in the concentration of hemoglobin-NO from 0.9 ± 0.2 to 4.8 ± 0.7 μM. This accumulation was attenuated by a nonselective inhibitor of NO synthase, NG-nitro-l-argininemethyl ester (l-NAME), but not by NG-nitro-d-argininemethyl ester (d-NAME) or 1400W. Administration of l-NAME (but not 1400W or d-NAME) during H produced a short-term increase in mean arterial pressure (∼90%). In H, the level of N oxides in red blood cells increased sevenfold. S-nitrosylation of plasma proteins was revealed with “biotin switch” techniques. The results provide compelling evidence that there is brisk production of NO in early H. The results indicate that the initial compensatory response to H is more complicated than previously realized, and it involves an orchestrated balance between intense vasoconstrictor and vasodilatory components. |
| Databáze: | OpenAIRE |
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