Rapid Detection of Infectious Flacherie Virus of the Silkworm,Bombyx mori, using RT-PCR and Nested PCR
| Autor: | Neetha Kari, Mallikarjun Gadwala, Shyam Kumar Vootla, Qineng Lu, Xing Meng Lu |
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| Rok vydání: | 2013 |
| Předmět: |
diagnosis
viruses Polymerase Chain Reaction Article Virus law.invention Bombycidae Bombyx mori law Animals RNA Viruses Polymerase chain reaction Bombyx biology Reverse Transcriptase Polymerase Chain Reaction fungi BmIFV General Medicine biology.organism_classification Virology Reverse transcriptase Real-time polymerase chain reaction Larva Insect Science RNA Viral Nested polymerase chain reaction |
| Zdroj: | Journal of Insect Science |
| ISSN: | 0970-3837 1536-2442 |
| DOI: | 10.1673/031.013.12001 |
| Popis: | In this study, a method for detection of an ssRNA viral pathogen that causes viral flacherie in the silkworm, Bombyx mori (L.) (Lepidoptera: Bombycidae), was used for the detection of B. mori infectious flacherie virus (BmIFV). A combination of nested and reverse transcriptase polymerase chain reaction was used for detection. Although BmIFV has been reported in almost all the sericultural regions of the world, there had been no reports of BmIFV incidence in India. Therefore, the confirmation of the presence of BmIFV in Karnataka, India, is of great significance. The present method is advantageous because it can be used to detect the virus by using samples from infected midgut tissues, thus simplifying and avoiding laborious genome isolation procedures. This method could help in early detection of BmIFV disease pathogens and help reduce crop losses. |
| Databáze: | OpenAIRE |
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