Redox cycling induces spermptosis and necrosis in stallion spermatozoa while the hydroxyl radical (OH center dot) only induces spermptosis
| Autor: | L. Anel-López, Mercedes Alvarez, P. de Paz, C. Ortega Ferrusola, P. Martin Muñoz, Luis Anel, José M. Ortiz-Rodríguez, Fernando J. Peña, C Balao da Silva, H. Rodriguez Martinez, María Concepción Robles Gil |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine endocrine system Fysiologi Physiology Apoptosis Mitochondrion Biology Dinoprost medicine.disease_cause Lipid peroxidation Andrology Necrosis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Menadione medicine Animals Ferrous Compounds Horses Membrane Potential Mitochondrial chemistry.chemical_classification Aldehydes Reactive oxygen species 030219 obstetrics & reproductive medicine Caspase 3 Hydroxyl Radical urogenital system Vitamin K 3 Spermatozoa Sperm Oxidative Stress 030104 developmental biology chemistry Biochemistry Sperm Motility Animal Science and Zoology Hydroxyl radical Lipid Peroxidation Oxidation-Reduction Oxidative stress Biotechnology |
| Popis: | Oxidative stress is a major factor explaining sperm dysfunction of spermatozoa surviving freezing and thawing and is also considered a major inducer of a special form of apoptosis, visible after thawing, in cryopreserved spermatozoa. To obtain further insights into the link between oxidative stress and the induction of apoptotic changes, stallion spermatozoa were induced to oxidative stress through redox cycling after exposure to 2-methyl-1,4-naphthoquinone (menadione), or hydroxyl radical formation after FeSO4 exposure. Either exposure induced significant increases (p amp;lt; 0.05) in two markers of lipid peroxidation: 8-iso-PGF(2) and 4-hydroxynonenal (4-HNE). While both treatments induced changes indicative of spermptosis (caspase-3 activation and decreased mitochondrial membrane potential) (p amp;lt; 0.01), menadione induced sperm necrosis and a dramatic reduction in motility and thiol content in stallion spermatozoa. Thus, we provided evidence that oxidative stress underlies spermptosis, and thiol content is a key factor for stallion sperm function. Funding Agencies|Secretaria de Estado de Investigacion, Desarrollo e Innovacion [AGL2013-43211-R, IJCI-2014-21671]; Ministerio de Educacion Cultura y Deporte [FPU13/03991]; Ministerio de Economia y Competitividad-FEDER [AGL2013-43211-R]; Junta de Extremadura-FEDER [IB16030, GR 15029]; Swedish Research Councils [521-2011-6353]; Formas [221-2011-512] |
| Databáze: | OpenAIRE |
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