Autoradiographic evidence for the sliding of the upper layer over the basement membrane in chicken blastoderms during gastrulation
Autor: | Marc Callebaut, H. Bortier, E. van Nueten, L. Vakaet |
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Rok vydání: | 2002 |
Předmět: |
Models
Anatomic animal structures Transplantation Heterologous Chick Embryo Coturnix Basement Membrane biology.animal Culture Techniques medicine Animals Blastoderm Basement membrane Polarity (international relations) biology Chemistry Primitive streak Chimera Anatomy Gastrula Agricultural and Biological Sciences (miscellaneous) Quail Cell biology Gastrulation medicine.anatomical_structure Epiblast embryonic structures Autoradiography Neural plate |
Zdroj: | European journal of morphology |
ISSN: | 0924-3860 |
Popis: | An upper layer (epiblast) fragment taken laterally from the Anlage fields of neural plate or chordamesoderm of a quail blastoderm, labelled with 3H-glucosamine, was grafted isotopically (in a similar region), isochronically (at the similar stage of development) and isotropically (with the same caudocranial and dorsoventral polarity) in the epiblast of a mesoblast free area of a chicken blastoderm (St 4-5 Vakaet, 1970: full grown primitive streak). On the autoradiographs of the sections through such cultured blastoderms with fully integrated quail grafts, we observed a labelling of the basement membrane laterally and slightly cranially from the labelled graft in its final position. Since only the epiblast and its basement membrane are involved, the pattern of the observed labelling indicates that the grafted and integrated quail epiblast fragment glides in toto over the mediocaudally localized basement membrane, leaving behind a track of radioactivity. Sliding of whole groups of epiblast cells over the basement membrane seems thus to be a normal phenomenon during avian gastrulation. |
Databáze: | OpenAIRE |
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