Intramolecular labelling of sucrose made by leaves from [14C)carbon dioxide or [3-14C]serine
| Autor: | I. F. Bird, M. J. Cornelius, C. P. Whittingham, Alfred J. Keys |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Sucrose
Chemical Phenomena chemistry.chemical_element Photosynthesis Biochemistry Serine chemistry.chemical_compound Hydrolysis Organic chemistry Carbon Radioisotopes Molecular Biology Triticum Plants Medicinal Fabaceae Cell Biology Metabolism Carbon Dioxide Plants Chemistry Invertase chemistry Carbon dioxide Carbon Nuclear chemistry Research Article |
| Zdroj: | The Biochemical journal. 172(1) |
| ISSN: | 0264-6021 |
| Popis: | Pea leaves were illuminated in air containing 150 or 1000p.p.m. of 14CO2 for various times. Alternatively, segments of wheat leaves were supplied with [3-14C]serine for 40 min in the light in air with 145, 326 or 944p.p.m. of 12CO2. Sucrose was extracted from the leaf material, hydrolysed with invertase, and 14C in the pairs of carbon atoms C-3+C-4, C-2+C-5 and C-1+C-6 in the glucose moiety was measured. The results obtained after metabolism of 14CO2 were consistent with the operation of the photosynthetic carbon-reduction cycle; the effects of CO2 concentration on distribution of 14C in the carbon chain of glucose after metabolism of [3-14C]serine is more easily explained by metabolism through the glycollate pathway than by the carbon-reduction cycle. |
| Databáze: | OpenAIRE |
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