LINE-1 is preferentially hypomethylated within adenomatous polyps in the presence of synchronous colorectal cancer
| Autor: | Faraz Bishesari, Lela Buckingham, Alice Chu Jiang, Joshua Melson, William Barbanera, Amoah Yeboah Korang |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Adenomatous polyps Colorectal cancer Gastroenterology LINE-1 03 medical and health sciences 0302 clinical medicine Internal medicine Genetics medicine In patient Molecular Biology Genetics (clinical) business.industry Research Cancer Methylation Field defect medicine.disease digestive system diseases 030104 developmental biology CpG site 030220 oncology & carcinogenesis DNA methylation business Global methylation Developmental Biology DNA hypomethylation |
| Zdroj: | Clinical Epigenetics |
| ISSN: | 1868-7075 |
| DOI: | 10.1186/s13148-017-0325-7 |
| Popis: | Background Conventional tubular adenomas are frequently detected in patients undergoing average risk screening colonoscopy and are over-represented in patients who will develop colorectal cancer (CRC). Whether features of adenomas could serve as predictors of synchronous CRC is not known. Here, we investigate whether global methylation markers, including LINE-1, differ within adenomas in patients with and without synchronous CRC. Methods Colorectal tubular/tubulovillous adenomatous polyps in the absence (P group, n = 45) and in the presence of synchronous CRC (PC group, n = 32) were identified. Global methylation and demethylation by ELISA for 5-methylcytosine (5-mC) and 5-hydroxymethyl cytosine (5-hmC), respectively, were assessed in polyps and adjacent normal non-neoplastic tissue. LINE-1 hypomethylation was assessed by pyrosequencing of bisulfite-converted DNA as well. Results Global methylation (5-mC) showed no differences in overall methylation status in the adenomatous polyps in the two groups (5-mC relative to control %, PC group 0.117; P group 0.161, p = 0.148). Global hydroxymethylation 5-hmC was also not significantly different in adenomatous polyps of the PC group than in those of the P group (0.0059 vs 0.0097, p = 0.681). Similarly, global 5-hmC was not different between normal tissues from patients without neoplasia in comparison to those from CRC patients (0.0461 ± 0.080 vs 0.039 ± 0.159, p = 0.215). In contrast, adenomatous polyps of the PC group had lower levels of LINE-1 methylation compared to the adenomas in the P group (53.07 ± 4.5 vs 59.95 ± 5.4, p |
| Databáze: | OpenAIRE |
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