High-throughput transfection of interfering RNA into a 3D cell-culture chip

Autor:	Michael G. Hogg, Susan T. Sharfstein, Jonathan S. Dordick, Moo-Yeal Lee, Haiyuan Zhang
Rok vydání:	2011
Předmět:	Cell Culture Techniques RNA General Chemistry Transfection Biology Molecular biology Cell biology Cell Line Biomaterials 3D cell culture Mice In vivo Cell culture Gene technology NIH 3T3 Cells Animals General Materials Science RNA Interference DNA microarray Gene Biotechnology
Zdroj:	Small (Weinheim an der Bergstrasse, Germany). 8(13)
ISSN:	1613-6829
Popis:	A method for high-throughput retroviral transfection of genes and interfering RNA into 3D cell-culture microarrays is described. 3D cultures more closely mimic the in vivo cellular milieu, thus providing cellular responses to genetic manipulation more similar to the in vivo situation than 2D cultures. This technique is applied to transfect several "toxic" short-hairpin RNAs (shRNAs) into 3D cell cultures. It is demonstrated that the toxicity is similar to that obtained by conventional (non-high-throughput) retroviral transfection of cells grown in similar 3D culture microarrays.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a2c16199e2f39cb0d4e7462b8389d0a9 https://pubmed.ncbi.nlm.nih.gov/22511323 Zobrazit plný text záznamu Plný text