Subventricular zone microglia possess a unique capacity for massivein vitroexpansion
| Autor: | Dennis A. Steindler, Meryem Demir, Eric D. Laywell, Gregory P. Marshall |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Neurogenesis
Phagocytosis Population Cell Culture Techniques Subventricular zone Nerve Tissue Proteins Cell Separation Biology Article Mice Cellular and Molecular Neuroscience Prosencephalon Immune system Lateral Ventricles medicine Animals education Cells Cultured Cell Proliferation education.field_of_study Microglia Cell growth Dissection Stem Cells Phenotype Mice Inbred C57BL medicine.anatomical_structure nervous system Neurology Neuroscience Biomarkers |
| Zdroj: | Glia. 56:1799-1808 |
| ISSN: | 1098-1136 0894-1491 |
| DOI: | 10.1002/glia.20730 |
| Popis: | Microglia, the resident immune cells of the brain, have recently been hypothesized to play a role both in neuronal diseases and age related neurogenic decline, and are theorized to be modulators of adult neurogenesis. Current methods for the isolation of microglia from cultured primary brain tissue result in relatively poor yield, requiring a large tissue sample or multiple specimens in order to obtain a sufficient number of microglia for cell and molecular analysis. We report here a method for the repetitive isolation of microglia from established glial monolayer cultures from which it is possible to expand the initial population of microglia roughly 10,000 fold. The expanded population expresses appropriate microglial morphology and phenotype markers, and demonstrates functionally normal phagocytosis, thus providing a high-yield assay for the investigation and analysis of microglia from a single initial dissection of primary tissue. Furthermore, this massive expansion is limited to microglia derived from the subventricular zone as the fold expansion of isolatable microglia was found to be up to 20 times greater than cultures from other brain regions, indicating unique properties for this persistently neurogenic region. |
| Databáze: | OpenAIRE |
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