Study of naïve and memory cells in a cohort of Egyptian chronic granulomatous disease patients
| Autor: | Dalia Abd Elaziz, Safa Meshaal, Nermeen Galal, Jeannette Boutros, Rabab El Hawary, Diana Nagy, Reem Jan Farid, Ingy Fikry, Radwa Alkady, Aisha Elmarsafy |
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| Rok vydání: | 2015 |
| Předmět: |
Male
congenital hereditary and neonatal diseases and abnormalities T-Lymphocytes CD3 Granulomatous Disease Chronic Biochemistry CD19 Cohort Studies Chronic granulomatous disease immune system diseases hemic and lymphatic diseases medicine Humans Child Memory B cell Molecular Biology B-Lymphocytes Cluster of differentiation biology business.industry Infant Cell Biology medicine.disease Acquired immune system Child Preschool Cohort Immunology biology.protein Primary immunodeficiency Egypt Female business |
| Zdroj: | Journal of Receptors and Signal Transduction. 35:423-428 |
| ISSN: | 1532-4281 1079-9893 |
| Popis: | Chronic granulomatous disease (CGD) is a primary immunodeficiency disorder caused by inherited defects in the NADPH oxidase complex which may be involved in important pathways that connect innate and adaptive immunity.Characterize the naive and memory compartment of B and T lymphocytes in patients with CGD.Twenty CGD patients and twenty healthy controls matched for age and sex were enrolled in this study. Flow cytometric assessment of the naïve and memory compartments of peripheral blood lymphocytes was done using cell surface markers CD45RO, CD45RA, CD27, CD3 and CD19.There were 15 (79%) autosomal recessive CGD patients (8 females (53%) and 7 males (47%), 100% positive parental consanguinity) and four (21%) X-linked CGD patients. On comparing the 3 groups; AR CGD, X-linked CGD and controls, there was a positive statistical significant difference for the percentage and absolute count of CD19 + CD27+ memory B cell (p = 0.028 and p = 0.047 respectively), CD45RA cells (with p values of p = 0.000 and 0.033, respectively), the naïve compartment CD3 + CD45RA+ cells percentage and absolute counts (p = 0.005, 0.01respectively), CD3 + CD27 + cells percentage and absolute counts (p = 0.001, 0.012 respectively), CD3 + CD45RA + CD27+ cells percentage and absolute counts (p = 0.015, 0.005, respectively). The significance was mainly attributed to the decrease in the X-linked group than control group.There was an altered naïve and memory B profile in CGD patients, this may increase susceptibility of the patients to opportunistic infections and autoimmune disorders. T-cell alterations have to be interpreted cautiously especially in the presence of infections. |
| Databáze: | OpenAIRE |
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