Temperature sensitivity on growth and/or replication of H1N1, H1N2 and H3N2 influenza A viruses isolated from pigs and birds in mammalian cells
| Autor: | Aurélie Oger, Stéphanie Bougeard, Estelle Marquet-Blouin, Céline Deblanc, Gaëlle Kuntz-Simon, Sylvie van der Werf, Pascale Massin, Cyril Barbezange, Véronique Jestin |
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| Přispěvatelé: | Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Génétique Moléculaire des Virus Respiratoires, Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Institut Pasteur [Paris], Institut Pasteur [Paris]-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Swine
viruses Biology medicine.disease_cause Virus Replication Microbiology Virus Cell Line Birds 03 medical and health sciences Dogs Influenza A Virus H1N1 Subtype Orthomyxoviridae Infections Influenza A Virus H1N2 Subtype Influenza Human Influenza A virus medicine Animals Humans [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology Phylogeny 030304 developmental biology Swine Diseases 0303 health sciences Genetic diversity General Veterinary 030306 microbiology Host (biology) Influenza A Virus H3N2 Subtype Intermediate host Temperature virus diseases General Medicine Viral Load Virology In vitro Influenza A virus subtype H5N1 3. Good health Cell culture Influenza in Birds Chickens |
| Zdroj: | Veterinary Microbiology Veterinary Microbiology, Elsevier, 2009, epub ahead of print. ⟨10.1016/j.vetmic.2009.10.012⟩ Veterinary Microbiology, 2009, epub ahead of print. ⟨10.1016/j.vetmic.2009.10.012⟩ |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2009.10.012⟩ |
| Popis: | International audience; Influenza A viruses have been isolated from a wide range of animal species, aquatic birds being the reservoir for their genetic diversity. Avian influenza viruses can be transmitted to humans, directly or indirectly through an intermediate host like pig. This study aimed to define in vitro conditions that could prove useful to evaluate the potential of influenza viruses to adapt to a different host. Growth of H1N1, H1N2 and H3N2 influenza viruses belonging to different lineages isolated from birds or pigs prior to 2005 was tested on MDCK or NPTr cell lines in the presence or absence of exogenous trypsin. Virus multiplication was compared at 33, 37 and 40 degrees C, the infection site temperatures in human, swine and avian hosts, respectively. Temperature sensitivity of PB2-, NP- and M-RNA replication was also tested by quantitative real-time PCR. Multiplication of avian viruses was cold-sensitive, whatever cell type. By contrast, temperature sensitivity of swine viruses was found to depend on the virus and the host cell: for an H1N1 swine isolate from 1982, multiplication was cold-sensitive on NPTr cells and undetectable at 40 degrees C. From genetic analyses, it appears that temperature sensitivity could involve other residues than PB2 residue 627 and could affect other steps of the replication cycle than replication. |
| Databáze: | OpenAIRE |
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