Establishment and characterization of a new human erythroleukemic cell line, ERY-1

Autor: Marie-Claude Piffaut, Laurence Leriche, Nadine Ben Hamouda, Viviane Tricottet, Hélène Merle-Béral, René Lai Kuen, Antoine Ribadeau Dumas, Michel Arock, Florence Nguyen Khac, Patrick Bonnemye
Přispěvatelé: Service d'hématologie biologique [CHU Pitié-Salpêtrière], Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Laboratoire de Biologie et de Pharmacologie Appliquée (LBPA), École normale supérieure - Cachan (ENS Cachan)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2003
Předmět:
Cancer Research
MESH: Piperazines
Fusion Proteins
bcr-abl
Chromosomal translocation
Trisomy
Piperazines
Translocation
Genetic
Fusion gene
MESH: Transforming Growth Factor beta1
0302 clinical medicine
Immunophenotyping
MESH: Aged
80 and over
Transforming Growth Factor beta
hemic and lymphatic diseases
Gene Duplication
Receptors
Erythropoietin
MESH: Antigens
CD
MESH: Aged
Aged
80 and over
0303 health sciences
medicine.diagnostic_test
MESH: Gene Duplication
Myeloid leukemia
MESH: Mast Cells
Hematology
MESH: Translocation
Genetic
Globins
Phenotype
Oncology
030220 oncology & carcinogenesis
Benzamides
Imatinib Mesylate
MESH: Trisomy
[SDV.IMM]Life Sciences [q-bio]/Immunology
Female
MESH: Leukemia
Erythroblastic
Acute
Chromosomes
Human
Pair 8
MESH: Cell Line
Tumor
MESH: Immunophenotyping
Biology
MESH: Phenotype
Transforming Growth Factor beta1
03 medical and health sciences
Antigens
CD
Cell Line
Tumor
Leukemia
Myelogenous
Chronic
BCR-ABL Positive
medicine
MESH: Globins
Humans
MESH: Receptors
Erythropoietin
Mitosis
MESH: Transforming Growth Factor beta
030304 developmental biology
MESH: Bone Marrow Examination
Aged
MESH: Humans
MESH: Fusion Proteins
bcr-abl
Molecular biology
Erythropoietin receptor
Pyrimidines
MESH: Leukemia
Myelogenous
Chronic
BCR-ABL Positive
Cell culture
MESH: Pyrimidines
MESH: Mastocytosis
Leukemia
Erythroblastic
Acute
MESH: Chromosomes
Human
Pair 8
MESH: Female
Fluorescence in situ hybridization
Zdroj: Leukemia Research
Leukemia Research, Elsevier, 2004, 28 (12), pp.1329-39. ⟨10.1016/j.leukres.2004.04.013⟩
ISSN: 0145-2126
DOI: 10.1016/j.leukres.2004.04.013⟩
Popis: The growth factor-independent erythroleukemic cell line ERY-1 was established from the peripheral blood of a 87-year-old woman with chronic myeloid leukemia (CML) in the acute phase. Immunophenotyping showed that fresh leukemic cells were positive for CD13, CD33, CD36 and CD235a (glycophorin A), a phenotype compatible with that of erythroblastic cells. Cytogenetic and fluorescence in situ hybridization (FISH) analysis demonstrated classical t(9;22)(q34;q11) chromosomic translocation associated with a duplication of the BCR-ABL fusion gene. Other cytogenetic abnormalities were detected in all analyzed mitosis, the most frequent being a trisomy of chromosome 8. The established ERY-1 cell line retains these immunophenotypic and cytogenetic features, and light and electron microscopy confirmed the relatively mature erythroblastic phenotype of the cells. In addition, ERY-1 cell line expressed beta-globin mRNA and a non-phosphorylable form of the erythropoietin receptor, even in presence of erythropoietin. Of note, the proliferation of ERY-1 cells was inhibited by TGFbeta1 or STI-571 (Gleevec), without significant induction of further differentiation. In conclusion, ERY-1 is a new growth factor-independent human erythroleukemic cell line with a relatively mature phenotype that may be useful to study the molecular events involved in erythroblastic differentiation.
Databáze: OpenAIRE
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