Elusive Recurrent Bacterial Contamination in a Diatom Culture: A Case Study
| Autor: | Darya P. Petrova, Artyom M. Marchenkov, Alexey A. Morozov, Yuri P. Galachyants, Yulia R. Zakharova |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Technology
QH301-705.5 In silico QC1-999 axenic cultures Genome 16S rRNA amplicon sequencing Microbiology diatoms Fragilaria contamination Sphingomonas General Materials Science Biology (General) Axenic Instrumentation QD1-999 Fluid Flow and Transfer Processes biology Process Chemistry and Technology Physics General Engineering Contamination biology.organism_classification 16S ribosomal RNA Engineering (General). Civil engineering (General) Computer Science Applications Chemistry Diatom TA1-2040 |
| Zdroj: | Applied Sciences, Vol 11, Iss 10131, p 10131 (2021) Applied Sciences Volume 11 Issue 21 |
| ISSN: | 2076-3417 |
| Popis: | In preparation for whole-genome sequencing, the axenic culture for two strains of a freshwater diatom Fragilaria radians were produced. Although their axenicity was controlled for the cultures’ entire lifetime, the published genomic assembly was later found to contain a large amount of bacterial sequences. Using various in silico analyses of whole genome read libraries and 16S rRNA sequencing of culture samples, we reconstruct the history of the contamination and document the failures of various axenicity control methods. This knowledge is used to discuss how these failures could have been avoided, and to provide guidelines for future works on axenic diatom cultures. |
| Databáze: | OpenAIRE |
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