Central neuropeptide Y receptors are involved in 3rd ventricular ghrelin induced alteration of colonic transit time in conscious fed rats
| Autor: | Silke Mronga, Clemens G Tebbe, Johannes J. Tebbe, Michael Ritter, Martin K.-H. Schäfer |
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| Jazyk: | angličtina |
| Předmět: |
Male
Receptors Neuropeptide medicine.medical_specialty medicine.drug_class Colon Peptide Hormones Central nervous system Stimulation Peptide hormone Arginine Receptors G-Protein-Coupled Rats Sprague-Dawley Orexigenic Internal medicine medicine Animals lcsh:RC799-869 Receptor Gastrointestinal Transit Injections Intraventricular Third Ventricle business.industry digestive oral and skin physiology Gastroenterology Brain General Medicine Receptor antagonist Neuropeptide Y receptor Ghrelin Rats Receptors Neuropeptide Y medicine.anatomical_structure Endocrinology lcsh:Diseases of the digestive system. Gastroenterology business Gastrointestinal Motility Injections Intraperitoneal hormones hormone substitutes and hormone antagonists medicine.drug Research Article |
| Zdroj: | BMC Gastroenterology, Vol 5, Iss 1, p 5 (2005) BMC Gastroenterology |
| ISSN: | 1471-230X |
| DOI: | 10.1186/1471-230x-5-5 |
| Popis: | Background Feeding related peptides have been shown to be additionally involved in the central autonomic control of gastrointestinal functions. Recent studies have shown that ghrelin, a stomach-derived orexigenic peptide, is involved in the autonomic regulation of GI function besides feeding behavior. Pharmacological evidence indicates that ghrelin effects on food intake are mediated by neuropeptide Y in the central nervous system. Methods In the present study we examine the role of ghrelin in the central autonomic control of GI motility using intracerobroventricular and IP microinjections in a freely moving conscious rat model. Further the hypothesis that a functional relationship between NPY and ghrelin within the CNS exists was addressed. Results ICV injections of ghrelin (0.03 nmol, 0.3 nmol and 3.0 nmol/5 μl and saline controls) decreased the colonic transit time up to 43%. IP injections of ghrelin (0.3 nmol – 3.0 nmol kg-1 BW and saline controls) decreased colonic transit time dose related. Central administration of the NPY1 receptor antagonist, BIBP-3226, prior to centrally or peripherally administration of ghrelin antagonized the ghrelin induced stimulation of colonic transit. On the contrary ICV-pretreatment with the NPY2 receptor antagonist, BIIE-0246, failed to modulate the ghrelin induced stimulation of colonic motility. Conclusion The results suggest that ghrelin acts in the central nervous system to modulate gastrointestinal motor function utilizing NPY1 receptor dependent mechanisms. |
| Databáze: | OpenAIRE |
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