Quantitative Determination of 15 Active Components in Lepidium meyenii with UHPLC-PDA and GC-MS
| Autor: | Long Cheng, Dao-Peng Tan, Zi-Qian Wang, Xing-Sha Mei, Meng-Yao Cui, Yao-Qing Xu, Hong Feng, San-Yang Qiao, Geng Li |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
QD71-142
Chromatography Article Subject Lepidium meyenii Chemistry Ammonium phosphate General Chemical Engineering Active components Repeatability Mass spectrometry Computer Science Applications Analytical Chemistry chemistry.chemical_compound Uhplc pda Gas chromatography–mass spectrometry Instrumentation Electron ionization Research Article |
| Zdroj: | Journal of Analytical Methods in Chemistry Journal of Analytical Methods in Chemistry, Vol 2021 (2021) |
| ISSN: | 2090-8873 2090-8865 |
| Popis: | In this study, a method using ultrahigh-performance liquid chromatography with photodiode array (UHPLC-PDA) was established and validated for the simultaneous quantification of 10 active components, including eight macamides and two glucosinolates, in Lepidium meyenii (maca). A gas chromatographic mass spectroscopy (GC-MS) method was used to determine the levels of three benzyl isothiocyanates and two sterols in maca. Liquid chromatographic separation was achieved on a Waters Acquity UHPLC HSS T3 column (2.1 mm × 100 mm, 1.8 μm) with gradient elution over 15 min. The mobile phase was (B) acetonitrile-(A) 10 mM aqueous ammonium phosphate, and the detection wavelength was 210 nm. The gas chromatographic separation was performed on an SH-Rxi-1 MS column, and the ionization mode was electron ionization (EI). Two methods were confirmed to have desirable precision (RSD |
| Databáze: | OpenAIRE |
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