Dynamics of foot-and-mouth disease virus replication in cells at different phases of the cell-division cycle
| Autor: | Ryan Waters, Lise Mazelet, John B. Bashiruddin, Z. Zhang, Claudia Doel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
General Veterinary
biology viruses Cell RNA Cell cycle biology.organism_classification Molecular biology lcsh:S1-972 In vitro Virus Reverse transcription polymerase chain reaction medicine.anatomical_structure Viral entry foot-and-mouth disease virus|cell cycle|replication parasitic diseases medicine Foot-and-mouth disease virus lcsh:Agriculture (General) General Agricultural and Biological Sciences Biotechnology |
| Zdroj: | Frontiers of Agricultural Science and Engineering, Vol 1, Iss 3, Pp 250-257 (2014) |
| ISSN: | 2095-7505 |
| Popis: | Foot-and-mouth-disease virus (FMDV) replicates in epithelial cells. The restriction of FMDV RNA to the basal cell layer of epithelia suggests a possible link between FMDV replication in vivo and the cell status. This paper describes in vitro studies in which FMDV infection was investigated in cells that were held at various cell division phases using cell cycle inhibitors. The results suggest that when cells were arrested at the G1 or G1/S phase, high levels of viral RNA were detected by quantitative real-time reverse transcription PCR and viral protein synthesis was observed by specific labeling techniques. In contrast, when cells were arrested at the G2/M phase, reduced or no viral RNA synthesis was detected. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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