Association between proinflammatory cytokines and lipid peroxidation in patients with severe dengue disease around defervescence
| Autor: | B. Agiesh Kumar, Rajendiran Soundravally, Bobby Zachariah, P R Narayanan, Tamilarasu Kadhiravan, Shripad A. Patil, C.C. Cleetus, S.L. Hoti |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Microbiology (medical)
Adult Male TNF-α/IFN-γ ratio Adolescent medicine.medical_treatment Dengue virus Biology medicine.disease_cause Dengue fever Proinflammatory cytokine Lipid peroxidation Dengue chemistry.chemical_compound Interferon-gamma Young Adult Malondialdehyde Lipid peroxides medicine Humans Interferon gamma Severe Dengue Aged DSS Tumor Necrosis Factor-alpha General Medicine Dengue Virus Middle Aged medicine.disease Oxidative Stress Cytokine Infectious Diseases chemistry Case-Control Studies Immunology Cytokines Female Lipid Peroxidation DHF Oxidative stress medicine.drug |
| Zdroj: | International Journal of Infectious Diseases. :68-72 |
| ISSN: | 1201-9712 |
| DOI: | 10.1016/j.ijid.2013.09.022 |
| Popis: | Summary Objectives Proinflammatory cytokines and the oxidative stress response are reported to be involved in dengue viral disease. The present study investigated the correlation of proinflammatory cytokines and lipid peroxidation with dengue severity. Methods Clinical samples from 27 dengue fever (DF) cases, 30 dengue haemorrhagic fever (DHF) cases, and 24 dengue shock syndrome (DSS) cases were studied around defervescence, along with samples from 30 healthy controls. Plasma samples were analysed for tumour necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ) by ELISA and for malondialdehyde (MDA) by thiobarbituric acid assay. Results Dengue-infected individuals had significantly higher levels of TNF-α, IFN-γ, and MDA in comparison to controls. The ratio of TNF-α to IFN-γ was significantly higher in DHF and DSS than in DF. A TNF-α/IFN-γ ratio value of 5.69 around defervescence predicted DHF and DSS with moderate accuracy and thus may serve as an indicator to study dengue severity. The study observed a significant positive correlation of lipid peroxides with TNF-α levels and the TNF-α/IFN-γ ratio in severe dengue cases. Conclusions We propose that the oxidative stress response induced by the dengue virus may trigger the inflammatory cytokine responses in dengue severity and thereby contributes to the pathogenesis of the disease; however the interplay between the oxidative response and inflammatory activity in disease virulence needs further study. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|