ESR imaging on a solid-tumor-bearing mouse using spin-labeled dextran
| Autor: | Tateaki Ogata, Shunsuke Kazama, Hisashi Yoshioka, Mika Tada, Tomohiro Ito, Kieko Saito, Hisayuki Tanizawa |
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| Rok vydání: | 2001 |
| Předmět: |
Ratón
medicine.medical_treatment Intraperitoneal injection Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Spin probe Cyclic N-Oxides Peritoneal cavity chemistry.chemical_compound Mice Nuclear magnetic resonance In vivo medicine Distribution (pharmacology) Animals Molecular Biology Chemistry Organic Chemistry Electron Spin Resonance Spectroscopy Dextrans General Medicine Neoplasms Experimental Molecular Weight medicine.anatomical_structure Dextran Female Spin Labels Molecular probe Biotechnology Half-Life |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 65(4) |
| ISSN: | 0916-8451 |
| Popis: | Imaging of a tumor with ESR was tried using two different types of spin probes, a low molecular weight spin probe, CPROXYL, and a polymer spin probe, TEMPO-DX. Spin probes were administered to a mouse bearing a solid tumor that was a transplanted Ehrlich's ascites carcinoma in the back, using two methods, conventional intraperitoneal injection and continuous intravenous injection with a micro-feeder. First, the accumulation of the probe was examined by X-band ESR. CPROXYL, which was administered to a mouse intraperitoneally, was exclusively retained in urine, showing that it was rapidly excreted into the bladder, while TEMPO-DX was absorbed from the peritoneal cavity with difficulty to the vessel. Using continuous intravenous injection, CPROXYL was also rapidly excreted, but it was confirmed that TEMPO-DX concentrated in tumor tissue because it has a long half-life in vivo. In addition, measurement of ESR imaging was done to measure the distribution of spin probes with continuous intravenous injection. The strongest spot of CPROXYL was observed on ESR images, showing the accumulation at the bladder, while the spot of TEMPO-DX was observed in the solid tumor of the back of the mouse. These results suggest that TEMPO-DX could stay much longer than a low molecular weight spin probe in vivo and concentrate at the tumor. TEMPO-DX may be useful for developing specific ESR imaging agents for tumor. |
| Databáze: | OpenAIRE |
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