Low dose of luteolin activates Nrf2 in the liver of mice at start of the active phase but not that of the inactive phase

Autor: Atsushi Makiyama, Yoko Yamashita, Hitoshi Ashida, Tomoya Kitakaze
Rok vydání: 2020
Předmět:
0301 basic medicine
Male
Metabolic Processes
Protein metabolism
Gene Expression
Artificial Gene Amplification and Extension
Pharmacology
medicine.disease_cause
environment and public health
Biochemistry
Polymerase Chain Reaction
Antioxidants
chemistry.chemical_compound
Mice
0302 clinical medicine
Active phase
Gene expression
NAD(P)H Dehydrogenase (Quinone)
Luteolin
Protein Metabolism
Liquid Chromatography
Mice
Inbred ICR
Multidisciplinary
Chromatographic Techniques
respiratory system
Inactive phase
Up-Regulation
Circadian Rhythms
Liver
Medicine
Research Article
NF-E2-Related Factor 2
Science
Research and Analysis Methods
03 medical and health sciences
Biological Clocks
medicine
Genetics
Animals
Circadian rhythm
Molecular Biology Techniques
Molecular Biology
Cell Nucleus
Biology and Life Sciences
Metabolism
Cell Biology
High Performance Liquid Chromatography
Oxidative Stress
030104 developmental biology
chemistry
Chronobiology
030217 neurology & neurosurgery
Oxidative stress
Heme Oxygenase-1
Zdroj: PLoS ONE
PLoS ONE, Vol 15, Iss 4, p e0231403 (2020)
ISSN: 1932-6203
Popis: A flavone luteolin has various health-promoting activities. Several studies reported that high dose of luteolin activates the Nrf2/ARE pathway in the liver. However, the effect of the low dose of luteolin that can be taken from a dietary meal on the Nrf2 activation remain unclear. It is expected that the flavonoid metabolism possesses a circadian rhythm, since nutritional metabolism processes daily cycle. In this study we investigated whether an administration affects the Nrf2 activation. ICR mice were orally administered 0.01-10 mg/kg body weight of luteolin once a day for 7 days at two time-points: at the start of active phase (ZT12) or at that of inactive phase (ZT0). Luteolin increased the nuclear translocation of Nrf2, resulting in the increases in its target gene products HO-1 and NQO1 at ZT12 but not at ZT0. The expression level of Nrf2 was lower at ZT12 than at ZT0 in the liver. We also found that the level of luteolin aglycon in the plasma is higher at ZT12 than at ZT0. These results suggest that the low dose of luteolin can activate Nrf2 pathway and the aglycon form of luteolin may mainly contribute to activate the Nrf2 pathway at ZT12 in the liver.
Databáze: OpenAIRE
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