Maturation ameloblasts of the porcine tooth germ do not express amelogenin

Autor: Makoto Fukae, Kazuyoshi Wakida, Takahiro Satoda, Takashi Uchida, Hidehiro Ozawa, James P. Simmer, Norio Amizuka, Chikage Murakami
Rok vydání: 1999
Předmět:
Zdroj: Histochemistry and Cell Biology. 111:297-303
ISSN: 1432-119X
0948-6143
DOI: 10.1007/s004180050360
Popis: Amelogenins are the most abundant constituent in the enamel matrix of developing teeth. Recent investigations of rodent incisors and molar tooth germs revealed that amelogenins are expressed not only in secretory ameloblasts but also in maturation ameloblasts, although in relatively low levels. In this study, we investigated expression of amelogenin in the maturation stage of porcine tooth germs by in situ hybridization and immunocytochemistry. Amelogenin mRNA was intensely expressed in ameloblasts from the differentiation to the transition stages, but was not detected in maturation stage ameloblasts. C-terminal specific anti-amelogenin antiserum, which only reacts with nascent amelogenin molecules, stained ameloblasts from the differentiation to the transition stages. This antiserum also stained the surface layer of immature enamel at the same stages. At the maturation stage, no immunoreactivity was found within the ameloblasts or the immature enamel. These results indicate that, in porcine tooth germs, maturation ameloblasts do not express amelogenins, suggesting that newly secreted enamel matrix proteins from the maturation ameloblast are not essential to enamel maturation occurring at the maturation stage.
Databáze: OpenAIRE
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