Maturation ameloblasts of the porcine tooth germ do not express amelogenin
Autor: | Makoto Fukae, Kazuyoshi Wakida, Takahiro Satoda, Takashi Uchida, Hidehiro Ozawa, James P. Simmer, Norio Amizuka, Chikage Murakami |
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Rok vydání: | 1999 |
Předmět: |
Male
Histology Swine Immunocytochemistry In situ hybridization Matrix (biology) Dental Enamel Proteins stomatognathic system Ameloblasts Animals Molecular Biology Amelogenin Enamel paint Chemistry Tooth Germ Cell Biology Anatomy Amelogenesis Cell biology stomatognathic diseases Medical Laboratory Technology visual_art visual_art.visual_art_medium Female Ameloblast Developmental biology |
Zdroj: | Histochemistry and Cell Biology. 111:297-303 |
ISSN: | 1432-119X 0948-6143 |
DOI: | 10.1007/s004180050360 |
Popis: | Amelogenins are the most abundant constituent in the enamel matrix of developing teeth. Recent investigations of rodent incisors and molar tooth germs revealed that amelogenins are expressed not only in secretory ameloblasts but also in maturation ameloblasts, although in relatively low levels. In this study, we investigated expression of amelogenin in the maturation stage of porcine tooth germs by in situ hybridization and immunocytochemistry. Amelogenin mRNA was intensely expressed in ameloblasts from the differentiation to the transition stages, but was not detected in maturation stage ameloblasts. C-terminal specific anti-amelogenin antiserum, which only reacts with nascent amelogenin molecules, stained ameloblasts from the differentiation to the transition stages. This antiserum also stained the surface layer of immature enamel at the same stages. At the maturation stage, no immunoreactivity was found within the ameloblasts or the immature enamel. These results indicate that, in porcine tooth germs, maturation ameloblasts do not express amelogenins, suggesting that newly secreted enamel matrix proteins from the maturation ameloblast are not essential to enamel maturation occurring at the maturation stage. |
Databáze: | OpenAIRE |
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