Soluble expression and purification of Brucella cell surface protein (BCSP31) of Brucella melitensis and preparation of anti-BCSP31 monoclonal antibodies
| Autor: | Lei Zhang, Xing An Wu, Cui Hong An, Yang Xin Sun, Zhi Kai Xu, Fang Lin Zhang, Wen Tao Bai |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
medicine.drug_class
Blotting Western Enzyme-Linked Immunosorbent Assay Brucella Bacillus subtilis Monoclonal antibody medicine.disease_cause Brucellosis law.invention Microbiology Mycobacterium tuberculosis Western blot law Brucella melitensis Genetics medicine Molecular Biology Escherichia coli DNA Primers biology medicine.diagnostic_test Chemistry Antibodies Monoclonal Membrane Proteins General Medicine biology.organism_classification Virology Recombinant DNA |
| Zdroj: | Molecular Biology Reports. 39:431-438 |
| ISSN: | 1573-4978 0301-4851 |
| DOI: | 10.1007/s11033-011-0755-9 |
| Popis: | Brucella cell surface protein (BCSP31) is potentially useful for diagnosing brucellosis. We aimed to establish a monoclonal antibody (MAb) against Brucella melitensis BCSP31 and to investigate its distribution in diagnosis. Soluble recombinant BCSP31 was successfully expressed and purified. Two MAbs (1F1 and 1E5) against B. melitensis BCSP31, effective in detecting both recombinant and cellular proteins, were obtained and characterized. The MAbs did not react with Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Mycobacterium tuberculosis, or Bacillus aeruginosus, but strongly reacted with BCSP31 and B. melitensis by ELISA and Western blot analysis. We also tested different Brucella species and brucellosis using the prepared anti-BCSP31 MAbs. BCSP31 and anti-BCSP31 MAbs may play important roles in future research in diagnosing brucellosis. |
| Databáze: | OpenAIRE |
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