Effect of FCCP on tight junction permeability and cellular distribution of ZO-1 protein in epithelial (MDCK) cells
| Autor: | Changxin Li, Mark J. Poznansky |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
Cell Membrane Permeability Intracellular pH Cell Biophysics Fluorescent Antibody Technique Oxidative phosphorylation Biology Biochemistry Epithelium Oxidative Phosphorylation Cell Line Dogs medicine Animals Tight junction Membrane Proteins Biological activity Cell Biology Hydrogen-Ion Concentration Phosphoproteins Cell biology Electrophysiology Intercellular Junctions medicine.anatomical_structure Permeability (electromagnetism) Cell culture Zonula Occludens-1 Protein |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Biomembranes. 1030:297-300 |
| ISSN: | 0005-2736 |
| DOI: | 10.1016/0005-2736(90)90306-9 |
| Popis: | The effect of the uncoupler of oxidative phosphorylation, FCCP (carbonylcyanide p-trifluoromethoxyphenylhydrazone), on the tight junction of Madin-Darby canine kidney cells was examined. FCCP induced an abrupt decrease in the transepithelial electrical resistance of the confluent monolayers over a period of 20 s. When FCCP was withdrawn from the incubation medium, the monolayer resistance recovered to close to the original level in less than 2 h. Staining of the tight junction-associated protein ZO-1 showed that the changes in transepithelial electrical resistance were accompanied by a diffusing of the protein away from cell peripheries and a reconcentration to the tight junction areas following resistance recovery. Intracellular pH was decreased by FCCP on a similar time-scale with no obvious changes in ATP levels over this time-course. These data suggest that the uncoupler FCCP has a profound effect on tight junction permeability and cellular distribution of the tight junction protein ZO-1 in the epithelial cells and that it probably acts by breaking down proton gradients and altering intracellular pH. |
| Databáze: | OpenAIRE |
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