Efficient generation of smooth muscle cells from adipose-derived stromal cells by 3D mechanical stimulation can substitute the use of growth factors in vascular tissue engineering

Autor: Lydia A.M. Bolhuis-Versteeg, Mojtaba Parvizi, André A. Poot, Martin C. Harmsen
Přispěvatelé: Restoring Organ Function by Means of Regenerative Medicine (REGENERATE), Biomaterials Science and Technology, Faculty of Science and Technology
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Adipose tissue-derived stromal cells (ASC)
Cell Culture Techniques
Adipose tissue
Vascular tissue engineering
Stimulation
02 engineering and technology
PHENOTYPE
Applied Microbiology and Biotechnology
Extracellular matrix
Tissue engineering
IR-103861
Transforming growth factor beta 1 (TGF-beta 1)
Smooth muscle cells (SMC)
POLY(TRIMETHYLENE CARBONATE) SCAFFOLDS
Cells
Cultured
Tissue Scaffolds
Chemistry
PROLIFERATION
Cell Differentiation
General Medicine
Anatomy
021001 nanoscience & nanotechnology
musculoskeletal system
Biomechanical Phenomena
Cell biology
DIFFERENTIATION
Adipose Tissue
cardiovascular system
Molecular Medicine
Poly(1
3-trimethylene carbonate) (PTMC)
0210 nano-technology
tissues
EXPRESSION
3-trimethylene carbonate) (PTMC)
Stromal cell
BONE-MARROW
Myocytes
Smooth Muscle
MESENCHYMAL STEM-CELLS
Transforming Growth Factor beta1
03 medical and health sciences
Poly(1
Cell Adhesion
AORTIC-ANEURYSMS
Humans
MODULATION
Cell adhesion
Cell Proliferation
Tissue Engineering
Mesenchymal stem cell
IN-VITRO
METIS-320206
030104 developmental biology
Cell culture
Stromal Cells
Zdroj: Biotechnology Journal, 11(7), 932-944. WILEY-V C H VERLAG GMBH
Biotechnology journal, 11(7), 932-944. Wiley-VCH Verlag
ISSN: 1860-6768
DOI: 10.1002/biot.201500519
Popis: Occluding artery disease causes a high demand for bioartificial replacement vessels. We investigated the combined use of biodegradable and creep-free poly (1,3-trimethylene carbonate) (PTMC) with smooth muscle cells (SMC) derived by biochemical or mechanical stimulation of adipose tissue-derived stromal cells (ASC) to engineer bioartificial arteries. Biochemical induction of cultured ASC to SMC was done with TGF-1 for 7d. Phenotype and function were assessed by qRT-PCR, immunodetection and collagen contraction assays. The influence of mechanical stimulation on non-differentiated and pre-differentiated ASC, loaded in porous tubular PTMC scaffolds, was assessed after culturing under pulsatile flow for 14d. Assays included qRT-PCR, production of extracellular matrix and scanning electron microscopy. ASC adhesion and TGF-1-driven differentiation to contractile SMC on PTMC did not differ from tissue culture polystyrene controls. Mesenchymal and SMC markers were increased compared to controls. Interestingly, pre-differentiated ASC had only marginal higher contractility than controls. Moreover, in 3D PTMC scaffolds, mechanical stimulation yielded well-aligned ASC-derived SMC which deposited ECM. Under the same conditions, pre-differentiated ASC-derived SMC maintained their SMC phenotype. Our results show that mechanical stimulation can replace TGF-1 pre-stimulation to generate SMC from ASC and that pre-differentiated ASC keep their SMC phenotype with increased expression of SMC markers.
Databáze: OpenAIRE
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