Extracellular flux analyses reveal differences in mitochondrial PBMC metabolism between high-fit and low-fit females
| Autor: | Joëlle J. E. Janssen, Bart Lagerwaard, Mojtaba Porbahaie, Arie G. Nieuwenhuizen, Huub F. J. Savelkoul, R. J. Joost van Neerven, Jaap Keijer, Vincent C. J. de Boer |
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| Rok vydání: | 2022 |
| Předmět: |
Adult
Adolescent Physiology Endocrinology Diabetes and Metabolism Celbiologie en Immunologie Oxygen consumption rate Young Adult Oxygen Consumption Physiology (medical) PBMC metabolism Humans Exercise Life Style VLAG hemic and immune systems Biomarker Aerobic fitness level Lifestyle Flow Cytometry Mitochondria Cell Biology and Immunology Human and Animal Physiology WIAS Fysiologie van Mens en Dier Leukocytes Mononuclear Physical Endurance Female Protons Extracellular Space Glycolysis |
| Zdroj: | American Journal of Physiology-Endocrinology and Metabolism 322 (2022) 2 American Journal of Physiology-Endocrinology and Metabolism, 322(2), E141-E153 |
| ISSN: | 1522-1555 0193-1849 |
| Popis: | Analyzing metabolism of peripheral blood mononuclear cells (PBMCs) can possibly serve as a cellular metabolic read-out for lifestyle factors and lifestyle interventions. However, the impact of PBMC composition on PBMC metabolism is not yet clear, neither is the differential impact of a longer-term lifestyle factor versus a short-term lifestyle intervention. We investigated the effect of aerobic fitness level and a recent exercise bout on PBMC metabolism in females. PBMCs from 31 young female adults divided into a high-fit (V̇o2peak≥ 47 mL/kg/min,n= 15) and low-fit (V̇o2peak≤ 37 mL/kg/min,n= 16) groups were isolated at baseline and overnight after a single bout of exercise (60 min, 70% V̇o2peak). Oxygen consumption rate (OCR) and glycolytic rate (GR) were measured using extracellular flux (XF) assays and PBMC subsets were characterized using fluorescence-activated cell sorting (FACS). Basal OCR, FCCP-induced OCR, spare respiratory capacity, ATP-linked OCR, and proton leak were significantly higher in high-fit than in low-fit females (allP< 0.01), whereas no significant differences in glycolytic rate (GR) were found (allP> 0.05). A recent exercise bout did not significantly affect GR or OCR parameters (allP> 0.05). The overall PBMC composition was similar between high-fit and low-fit females. Mitochondrial PBMC function was significantly higher in PBMCs from high-fit than from low-fit females, which was unrelated to PBMC composition and not impacted by a recent bout of exercise. Our study reveals a link between PBMC metabolism and levels of aerobic fitness, increasing the relevance of PBMC metabolism as a marker to study the impact of lifestyle factors on human health. |
| Databáze: | OpenAIRE |
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