Tracking Immature Testicular Tissue after Vitrification In Vitro and In Vivo for Pre-Pubertal Fertility Preservation: A Translational Transgenic Mouse Model
| Autor: | Buo-Jia Lu, Ya-Li Huang, Yung-Liang Liu, Brian Shiian Chen, Bou-Zenn Lin, Chi-Huang Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Cryopreservation
Male pre-pubertal fertility preservation immature testicular tissue (ITT) solid surface vitrification (SSV) bioluminescence imaging (BLI) quantum yield (QY) Organic Chemistry Fertility Preservation Mice Transgenic General Medicine Vitrification Catalysis Computer Science Applications Inorganic Chemistry Disease Models Animal Mice Testis Animals Humans Physical and Theoretical Chemistry Spermatogenesis Molecular Biology Spectroscopy |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 15; Pages: 8425 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms23158425 |
| Popis: | Pediatric cancer survivors experiencing gonadotoxic chemoradiation therapy may encounter subfertility or permanent infertility. However, previous studies of cryopreservation of immature testicular tissue (ITT) have mainly been limited to in vitro studies. In this study, we aim to evaluate in vitro and in vivo bioluminescence imaging (BLI) for solid surface-vitrified (SSV) ITT grafts until adulthood. The donors and recipients were transgenic and wild-type mice, respectively, with fresh ITT grafts used as the control group. In our study, the frozen ITT grafts remained intact as shown in the BLI, scanning electron microscopy (SEM) and immunohistochemistry (IHC) analyses. Graft survival was analyzed by BLI on days 1, 2, 5, 7, and 31 after transplantation. The signals decreased by quantum yield between days 2 and 5 in both groups, but gradually increased afterwards until day 31, which were significantly stronger than day 1 after transplantation (p = 0.008). The differences between the two groups were constantly insignificant, suggesting that both fresh and SSV ITT can survive, accompanied by spermatogenesis, until adulthood. The ITT in both groups presented similar BLI intensity and intact cells and ultrastructures for spermatogenesis. This translational model demonstrates the great potential of SSV for ITT in pre-pubertal male fertility preservation. |
| Databáze: | OpenAIRE |
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