Phenotypic study of peripheral blood lymphocytes and humoral immune response in Helicobacter pylori infection according to age
| Autor: | Olindo Assis Martins-Filho, Gifone A. Rocha, Rodrigo Correa-Oliveira, Dulciene M.M. Queiroz, Anfrisina S. T. Carvalho, C. A. Oliveira, Paulo Fernando Souto Bittencourt, Taciana F. Soares, Andreia Maria Camargos Rocha, Ana Maria Caetano Faria |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Adult
Male Adolescent T-Lymphocytes Immunology Biology CD19 Helicobacter Infections Immunophenotyping Immune system Antigen Antigens CD Humans Child Aged B-Lymphocytes Helicobacter pylori Age Factors CD28 General Medicine Middle Aged biology.organism_classification Antibodies Bacterial Lymphocyte Subsets Child Preschool Immunoglobulin G biology.protein Female Antibody CD8 |
| Zdroj: | Scandinavian journal of immunology. 62(1) |
| ISSN: | 0300-9475 |
| Popis: | There are differences between children and adults in certain aspects of the Helicobacter pylori (HP) infection, among them the lower titre of IgG antibodies anti-HP in the former group. Thus, we investigated by means of flow cytometry CD4+/CD3+ (CD4+T), CD8+/CD3+ (CD8+T) and CD19+/CD3- (B) cells, activation/co-stimulatory markers (CD4+/HLA-DR+, CD4+/CD28+, CD8+/HLA-DR+ and CD8+/CD28+) and by means of ELISA IgG anti-HP antibodies in the peripheral blood from HP-positive and -negative children and adults. An increased CD4+/CD28+ and CD8+/CD28+ percentage and number of CD4+/CD3+ cells were seen in infected adults. Conversely, no difference was observed between infected and noninfected children, but when they were stratified by age, an increased CD4+/CD28+ cell percentage was seen in the HP-positive group older than 10 years. The mean level of IgG anti-HP was lower in younger infected children, increased with age and correlated with CD4+ cells. Our data suggest that the immune response to HP infection vary according to the age. Low percentage of activated CD4+ cell may contribute to the lower level of serum IgG anti-HP observed in younger infected children. In addition, the CD4+ cell participation during the infection seems to begin after 10 years old, when the immune response becomes similar to that seen in adults. |
| Databáze: | OpenAIRE |
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