Determination of Mycophenolic acid in the vitreous humor using the HPLC–ESI-MS/MS method: Application of intraocular pharmacokinetics study in rabbit eyes with ophthalmic implantable device
| Autor: | Gustavo de Oliveira Fulgêncio, Gerson Antônio Pianetti, Paula Rocha Chellini, Ricardo Martins Duarte Byrro, Armando da Silva Cunha |
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| Rok vydání: | 2013 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization genetic structures Clinical Biochemistry Pharmaceutical Science Hplc esi ms ms Pharmacology Tandem mass spectrometry Mycophenolic acid Analytical Chemistry Drug Delivery Systems Pharmacokinetics Tandem Mass Spectrometry Absorbable Implants Drug Discovery medicine Animals Chromatography High Pressure Liquid Spectroscopy Chromatography Chemistry Rabbit (nuclear engineering) Mycophenolic Acid Controlled release eye diseases Bioavailability Vitreous Body Drug delivery Female Rabbits sense organs Immunosuppressive Agents medicine.drug |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 84:30-35 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/j.jpba.2013.05.030 |
| Popis: | Mycophenolic acid (MPA) is an immunosuppressive agent widely used in the treatment of solid organ transplant rejection. The success of MPA in the treatment of inflammatory intraocular diseases has been reported in recent literature. The treatment of inflammatory eye diseases in the posterior chamber is a challenge due to the anatomy of the eye, which presents certain barriers to drug access. Thus, the bioavailability of drugs in the eye is quite low, and successful drug delivery may well represent a key limiting factor to attaining a successful therapeutic strategy. Ophthalmic controlled drug delivery offers the potential to enhance the efficacy of treatment for pathological conditions. Thus, a novel delivery system based on a biodegradable polymeric device, which can be implanted inside the eye and deliver MPA directly to the target, is being developed. Specific analytical methods to determine the use of effective drugs within the eye are needed to characterize this device. A liquid chromatography–electrospray ionization tandem mass spectrometry (HPLC–ESI-MS/MS) method for the quantitation of MPA in the vitreous humor of rabbits was developed and validated. The vitreous was collected from rabbits, extracted by a protein precipitation extraction procedure and then separated on a C 18 column with a mobile phase comprised of 0.15% aqueous acetic acid and methanol (60:40, v/v). The calibration curve was constructed within the range of 3–10,000 ng/mL for MPA. The mean R.S.D. values for the intra-run and inter-run precision were 5.15% and 4.35%. The mean accuracy value was 100.16%. The validated method was successfully applied to determine the MPA concentration in the vitreous humor of rabbits treated with an ocular implantable device. |
| Databáze: | OpenAIRE |
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