Characterization of microRNAs differentially expressed during bovine follicle development
| Autor: | Sadanand D Sontakke, Alan S. McNeilly, F. Xavier Donadeu, Bushra T Mohammed |
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| Rok vydání: | 2014 |
| Předmět: |
Embryology
Granulosa Cells Microarray Gene Expression Profiling Gene Expression Regulation Developmental Obstetrics and Gynecology Ovary Cell Biology Biology Oocyte Follicular cell Gene expression profiling Andrology MicroRNAs Follicle Endocrinology medicine.anatomical_structure Ovarian Follicle Reproductive Medicine microRNA medicine Animals Cattle Female Ovarian follicle |
| Zdroj: | REPRODUCTION. 148:271-283 |
| ISSN: | 1741-7899 1470-1626 |
| Popis: | Several different miRNAs have been proposed to regulate ovarian follicle function; however, very limited information exists on the spatiotemporal patterns of miRNA expression during follicle development. The objective of this study was to identify, using microarray, miRNA profiles associated with growth and regression of dominant-size follicles in the bovine monovular ovary and to characterize their spatiotemporal distribution during development. The follicles were collected from abattoir ovaries and classified as small (4–8 mm) or large (12–17 mm); the latter were further classified as healthy or atretic based on estradiol and CYP19A1 levels. Six pools of small follicles and individual large healthy (n=6) and large atretic (n=5) follicles were analyzed using Exiqon's miRCURY LNA microRNA Array 6th gen, followed by qPCR validation. A total of 17 and 57 sequences were differentially expressed (greater than or equal to twofold; P |
| Databáze: | OpenAIRE |
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