Isolation of copper-tolerant mutants of sake yeast with defective peptide uptake
Autor: | Shodo Hara, Haruhiko Mizoguchi, Keiji Furukawa, Tasuku Yamada |
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Rok vydání: | 2005 |
Předmět: |
Saccharomyces cerevisiae Proteins
Ubiquitin-Protein Ligases Molecular Sequence Data Saccharomyces cerevisiae Mutant Drug Resistance Angiotensin-Converting Enzyme Inhibitors Bioengineering Peptide Peptidyl-Dipeptidase A medicine.disease_cause Applied Microbiology and Biotechnology chemistry.chemical_compound Renin–angiotensin system medicine Sodium lactate Homeodomain Proteins chemistry.chemical_classification Mutation Base Sequence biology Alcoholic Beverages technology industry and agriculture Membrane Transport Proteins food and beverages biology.organism_classification Yeast Protein Transport Enzyme chemistry Biochemistry Fermentation Peptides Copper Transcription Factors Biotechnology |
Zdroj: | Journal of Bioscience and Bioengineering. 100:460-465 |
ISSN: | 1389-1723 |
DOI: | 10.1263/jbb.100.460 |
Popis: | Mutants of Saccharomyces cerevisiae that have a decreased peptide uptake ability were isolated from sake yeast. A copper medium containing copper sulfate, histidylleucine and sodium lactate as a carbon source was used for the isolation. One mutant showed a decreased peptide uptake ability due to PTR2 transcriptional repression, but for other mutants, the decrease was not due to the transcriptional repression. The peptide concentration in sake brewed with these mutants increased to about 1.5-fold that of sake brewed with the parental strain. The angiotensin I-converting enzyme (ACE) inhibitory activity of sake brewed with the mutant was about 3.6-fold that of sake brewed with the parental strain. |
Databáze: | OpenAIRE |
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