Analysis of ovarian transcriptomes reveals thousands of novel genes in the insect vector Rhodnius prolixus
Autor: | Ingrid Alexandre de Abreu Brito, Mateus Berni, Helena Araujo, Attilio Pane, Maira Arruda Cardoso, Michael Sammeth, Vitor Lima Coelho, Tarcísio Fontenele de Brito |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Molecular biology Trypanosoma cruzi Science Genome Insect De novo transcriptome assembly Biology Genome Oogenesis Article Germline Transcriptome 03 medical and health sciences 0302 clinical medicine Developmental biology Animals Humans Chagas Disease Transcriptomics Rhodnius prolixus Gene Genetics Multidisciplinary Gene Expression Profiling Ovary biology.organism_classification Insect Vectors Computational biology and bioinformatics Open reading frame 030104 developmental biology Gene Expression Regulation Rhodnius Medicine Female 030217 neurology & neurosurgery |
Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-17 (2021) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Rhodnius prolixus is a Triatominae insect species and a primary vector of Chagas disease. The genome of R. prolixus has been recently sequenced and partially assembled, but few transcriptome analyses have been performed to date. In this study, we describe the stage-specific transcriptomes obtained from previtellogenic stages of oogenesis and from mature eggs. By analyzing ~ 228 million paired-end RNA-Seq reads, we significantly improved the current genome annotations for 9206 genes. We provide extended 5′ and 3′ UTRs, complete Open Reading Frames, and alternative transcript variants. Strikingly, using a combination of genome-guided and de novo transcriptome assembly we found more than two thousand novel genes, thus increasing the number of genes in R. prolixus from 15,738 to 17,864. We used the improved transcriptome to investigate stage-specific gene expression profiles during R. prolixus oogenesis. Our data reveal that 11,127 genes are expressed in the early previtellogenic stage of oogenesis and their transcripts are deposited in the developing egg including key factors regulating germline development, genome integrity, and the maternal-zygotic transition. In addition, GO term analyses show that transcripts encoding components of the steroid hormone receptor pathway, cytoskeleton, and intracellular signaling are abundant in the mature eggs, where they likely control early embryonic development upon fertilization. Our results significantly improve the R. prolixus genome and transcriptome and provide novel insight into oogenesis and early embryogenesis in this medically relevant insect. |
Databáze: | OpenAIRE |
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