The effect of caspase inhibitors and neurotrophic factors on damaged retinal ganglion cells
| Autor: | Emiko Adachi-Usami, Toshiyuki Oshitari |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Retinal Ganglion Cells
Programmed cell death Neurite Biology Ciliary neurotrophic factor Retinal ganglion Mice Neurotrophic factors Neurites medicine Animals Nerve Growth Factors Enzyme Inhibitors Cells Cultured Retina Cell Death General Neuroscience Caspase Inhibitors eye diseases Nerve Regeneration Cell biology Mice Inbred C57BL medicine.anatomical_structure Nerve growth factor Retinal ganglion cell Caspases Immunology biology.protein sense organs |
| Zdroj: | NeuroReport. 14:289-292 |
| ISSN: | 0959-4965 |
| DOI: | 10.1097/00001756-200302100-00027 |
| Popis: | To elucidate the role of caspase inhibitors and neurotrophic factors in retinal ganglion cell (RGC) death and regeneration, we cultured mouse retinal explants in the presence of caspase-1, -3, -8, or -9 inhibitors, brain-derived neurotrophic factor (BDNF) and ciliary neurotrophic factor (CNTF) in serum-free culture media. We quantified apoptosis by TUNEL staining in RGCs and assessed the number of regenerating neurites. Apoptosis of RGCs treated with all caspase inhibitors or with neurotrophic factors was significantly reduced and the number of regenerating neurites was significantly greater than controls (p < 0.05). Our findings indicate that caspase-1, -3, -8, -9 play a critical role in explanted RGC death and may be ideal targets of neuroprotection and regeneration of damaged RGCs. |
| Databáze: | OpenAIRE |
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