Demonstration of antibodies to bovine desmocollin isoforms in certain pemphigus sera
| Autor: | Kevin K M Yue, R. W. Wilkinson, Takashi Hashimoto, Marian Dmochowski, David R. Garrod, Martyn A.J. Chidgey, Takeji Nishikawa |
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| Rok vydání: | 1995 |
| Předmět: |
Recombinant Fusion Proteins
Immunoblotting Dermatology Desmocollins Biology immune system diseases Desmosome medicine Animals Humans Fluorescent Antibody Technique Indirect skin and connective tissue diseases Pemphigus foliaceus chemistry.chemical_classification integumentary system Pemphigus vulgaris technology industry and agriculture Desmosomes medicine.disease Molecular biology Cytoskeletal Proteins Pemphigus medicine.anatomical_structure Desmoplakins chemistry Immunoglobulin G Immunology biology.protein Cattle Desmocollin Antibody Glycoprotein |
| Zdroj: | British Journal of Dermatology. 133:519-525 |
| ISSN: | 1365-2133 0007-0963 |
| DOI: | 10.1111/j.1365-2133.1995.tb02698.x |
| Popis: | We have shown previously that IgG antibodies in certain pemphigus sera, particularly endemic Brazilian pemphigus foliaceus (BPF) sera, react with bovine desmocollins (Dsc), which are transmembranous glycoproteins of desmosome junctions. Desmocollins occur as three different isoforms (Dsc 1, 2 and 3), all of which are represented in the epidermis. In this study, we examined sera of various pemphigus types by immunoblotting purified bovine desmosomes and bovine Dsc 1, 2 and 3 fusion proteins, expressed in pGEX expression vectors. Six of 15 (40.0%) BPF sera, two of 18 (11.1%) non-endemic pemphigus foliaceus sera, eight of 39 (20.5%) pemphigus vulgaris (PV) sera, and two of 11 (18.2%) normal sera, showed reactivity with Dsc from desmosomes. Experiments with fusion proteins showed that no Dsc isoform was specifically recognized by sera of any individual pemphigus type. Our results indicate that the pathogenesis of pemphigus might be more complex than previously believed. |
| Databáze: | OpenAIRE |
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