Shewanella fodinae sp. nov., isolated from a coal mine and from a marine lagoon
| Autor: | R. Sravan Kumar, Chi-Nam Seong, Chae Hong Lim, Ch. V. Ramana, Ch. Sasikala, Sungmin Park, T. S. Sasi Jyothsna |
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| Rok vydání: | 2010 |
| Předmět: |
Base Composition
Shewanella Base Sequence Phylogenetic tree Molecular Sequence Data General Medicine Biology biology.organism_classification 16S ribosomal RNA Microbiology Mining Coal Vibrionaceae RNA Ribosomal 16S Casein Shewanella haliotis Seawater Taxonomy (biology) Phylogeny Ecology Evolution Behavior and Systematics Bacteria |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 60:1649-1654 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijs.0.017046-0 |
| Popis: | Strains JC15T and JC19 were isolated from samples collected from different locations in India, including a coal mine and a marine lagoon. Both strains were Gram-stain-negative rods, motile by means of a single polar flagellum, catalase- and oxidase-positive, and hydrolysed casein, produced H2S and showed β-haemolysis. Strain JC15T grew optimally at pH 6 (range pH 5–8) while strain JC19 grew optimally at pH 7 (range pH 6-9) and both had a growth temperature optimum of 30–37 °C (range 22–40 °C). Both strains could grow chemo-organoheterotrophically and chemolithoautotrophically. Neither strain required NaCl for growth and both could tolerate up to 9 % (w/v) NaCl, with optimum growth at 5 % NaCl. Vitamin B12 was required as a growth factor by both strains. The major fatty acids were iso-C15 : 0, C17 : 1 ω8c and iso-C13 : 0 3-OH. The DNA G+C contents of strains JC15T and JC19 were 53.6 and 54.3 mol%, respectively. A phylogenetic tree based on 16S rRNA gene sequence analysis showed that strains JC15T and JC19 were most closely related to Shewanella haliotis DW01T (approximately 94 % sequence similarity) and to other members of the genus Shewanella. Genomic relatedness (DNA–DNA hybridization) between strains JC15T and JC19 is 88 %. On the basis of phenotypic and molecular genetic evidence, strain JC15T represents a novel species of the genus Shewanella, for which the name Shewanella fodinae sp. nov. is proposed. The type strain is JC15T (=CCUG 57102T =NBRC 105216T =KCTC 22506T). |
| Databáze: | OpenAIRE |
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