Faecal bile acids and colonic bile acid membrane receptor correlate with symptom severity of diarrhoea-predominant irritable bowel syndrome: A pilot study
| Autor: | Shu-kun Yao, Bingyu Niu, Hui-Fen Wang, Shuo Chen, Wei Wei, Wenxue Zhang, Yu Zhang, Yanli Zhang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Adult
Diarrhea Male medicine.medical_specialty Abdominal pain medicine.drug_class education Pilot Projects Severity of Illness Index Calcitriol receptor Gastroenterology Receptors G-Protein-Coupled Bile Acids and Salts Irritable Bowel Syndrome Feces 03 medical and health sciences 0302 clinical medicine Surveys and Questionnaires Internal medicine medicine Humans Receptor Irritable bowel syndrome Aged Hepatology Bile acid business.industry digestive oral and skin physiology Middle Aged medicine.disease G protein-coupled bile acid receptor Gastrointestinal Microbiome Cross-Sectional Studies Case-Control Studies 030220 oncology & carcinogenesis Receptors Calcitriol Immunohistochemistry Female 030211 gastroenterology & hepatology medicine.symptom business |
| Zdroj: | Digestive and Liver Disease. 53:1120-1127 |
| ISSN: | 1590-8658 |
| Popis: | Aims To compare both the faecal bile acids (BAs) and the levels of two bile acid receptors, Takeda G protein-coupled receptor 5 (TGR5) and vitamin D receptor (VDR), in the colonic mucosa between patients with irritable bowel syndrome with predominant diarrhea (IBS-D) and healthy controls, and explore the correlations among clinical characteristics, bile acid receptors expression, and BAs. Methods The severity of abdominal pain and diarrhoea was assessed in IBS-D patients using validated questionnaires, faecal BAs were measured by ultraperformance liquid chromatography coupled to tandem mass spectrometry, and rectosigmoid biopsies were taken for the analyses of TGR5 and VDR expression using immunohistochemistry. Results The level of TGR5 immunoreactivity in rectosigmoid mucosal biopsies was significantly higher in IBS-D patients than in controls, while the VDR immunoreactivity displayed no significant difference between patients and controls. The patients with more severe or more frequent abdominal pain had significantly higher TGR5 level. Faecal primary BAs were significantly increased in IBS-D patients and were positively correlated with the severity of diarrhoea. The level of TGR5 was positively associated with primary BAs and negatively associated with secondary BAs among all participants providing both mucosal and stool samples. Conclusions Colonic mucosal TGR5 protein expression and faecal bile acids were correlated with the symptom severity of IBS-D patients. |
| Databáze: | OpenAIRE |
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