Gene delivery using dendrimer/pDNA complexes immobilized in electrospun fibers using the Layer-by-Layer technique
| Autor: | Rita Castro, João Rodrigues, Pedro Filipe Pires, Helena Tomás, Kirthiga Ramalingam, Shili Xiao, Xiangyang Shi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Electrospun fibers
General Chemical Engineering Nanotechnology 02 engineering and technology General Chemistry Gene delivery 010402 general chemistry 021001 nanoscience & nanotechnology Layer-by-layer technique 01 natural sciences Polyelectrolyte Electrospinning Gene delivery using dendrimer/pDNA complexes 0104 chemical sciences Chitosan chemistry.chemical_compound PLGA Faculdade de Ciências Exatas e da Engenharia chemistry Tissue engineering Dendrimer Biophysics Surface modification 0210 nano-technology |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | A gene delivery platform for potential use in tissue engineering applications was developed by surface functionalization of biodegradable electrospun poly(lactic-co-glycolic acid) (PLGA) fibers with nanolayers of chitosan (cationic polymer) and alginate (anionic polymer) using the Layer-by-Layer (LbL) technique. The developed system not only supported the attachment and growth of human Mesenchymal Stem Cells (hMSCs), but also was capable of delivering pDNA/dendrimer complexes and inducing cell differentiation towards the osteogenic lineage when a pDNA codifying for human Bone Morphogenetic Protein-2 (BMP-2) was used. Beyond providing a means for pDNA/dendrimer complex immobilization, the polyelectrolyte coating conferred sustained release properties to the scaffold that resulted in pDNA protection from degradation. The polyelectrolyte coating, by itself, also contributed to enhance cell differentiation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|