Precise measurement of intradermal fluid delivery using a low activity technetium-99m pertechnetate tracer
| Autor: | Sahan A. Ranamukhaarachchi, Mehrsa Raeiszadeh, Boris Stoeber, Urs O. Häfeli, Tullio V F Esposito |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Injections
Intradermal Pertechnetate Swine medicine.medical_treatment 030231 tropical medicine Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Drug Delivery Systems 0302 clinical medicine In vivo TRACER medicine Volume of fluid method Animals 030212 general & internal medicine Saline Skin Sodium Pertechnetate Tc 99m Technetium 99m pertechnetate Vaccines General Veterinary General Immunology and Microbiology Public Health Environmental and Occupational Health Low activity Rats Infectious Diseases chemistry Needles Molecular Medicine Female Radiopharmaceuticals Ex vivo Biomedical engineering |
| Zdroj: | Vaccine. 37:7463-7469 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2019.09.078 |
| Popis: | A method was developed and validated to determine the intradermal (ID) fluid delivery potential of several ID devices, including hollow microneedles. The novel method used water soluble technetium-99 m pertechnetate (99mTcO4−) diluted in normal saline to measure the volume of fluid delivered to and remaining in the skin. The fluid that back-flowed to the skin surface and the fluid left on the device surface were also quantified, thus capturing all fluid volumes deposited during intradermal injections. The technique described in this manuscript was used to assess the injection performance of conventional hypodermic needles and hollow microneedles ex vivo using porcine skin and in vivo with a rat model. Since only a small fraction, 1.1%, of the water-soluble tracer remained bound to the skin when applied topically, the technique can be used to differentiate between injected fluid and backflow. Counting of gamma radiation from 99mTcO4− provided sub-nanoliter resolution for volume measurements, making the proposed method powerful, sensitive, and suitable for the assessments of ID injection devices, particularly for vaccine delivery. |
| Databáze: | OpenAIRE |
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