Arginine-modified DNA aptamers that show enantioselective recognition of the dicarboxylic acid moiety of glutamic acid
| Autor: | Kazuomi Ohsawa, Toshiyuki Kasamatsu, Hiroaki Ozaki, Hiroaki Sawai, Kazuo Hanawa, Masayasu Kuwahara, Jun-ichi Nagashima |
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| Rok vydání: | 2008 |
| Předmět: |
chemistry.chemical_classification
Magnetic Resonance Spectroscopy Arginine Stereochemistry Reverse Transcriptase Polymerase Chain Reaction Aptamer Stereoisomerism Glutamic acid Aptamers Nucleotide Surface Plasmon Resonance Chromatography Affinity Analytical Chemistry Dicarboxylic acid chemistry Biochemistry Glutamates Moiety Dicarboxylic Acids Spectrophotometry Ultraviolet Surface plasmon resonance Systematic evolution of ligands by exponential enrichment |
| Zdroj: | Analytical sciences : the international journal of the Japan Society for Analytical Chemistry. 24(1) |
| ISSN: | 0910-6340 |
| Popis: | We have screened glutamic acid-binding aptamers from a modified DNA pool containing arginine residues using the method of systematic evolution of ligands by exponential enrichment (SELEX). Thirty-one modified DNA molecules were obtained from the enriched pool after the 17th round of selection, and their binding affinities for the target were evaluated by binding assays using affinity gels. Three modified DNA molecules having higher affinity were sequenced and we determined their affinity and specificity for the target by surface plasmon resonance (SPR) measurements. The SPR studies indicated that two of these three aptamers distinguished the dicarboxylic acid moiety of the D-isomer from that of the L-isomer; however, the third aptamer did not show enantioselectivity. |
| Databáze: | OpenAIRE |
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