Acute lung injury and lung transplantation influence in vitro subtype conversion of pulmonary surfactant
| Autor: | Kevin Inchley, James F. Lewis, Ruud A. W. Veldhuizen, Richard J. Novick, Gayatri Maitra, Fred Possmayer |
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| Rok vydání: | 2002 |
| Předmět: |
Lung Diseases
Pulmonary and Respiratory Medicine Pulmonary Circulation Pathology medicine.medical_specialty 1 2-Dipalmitoylphosphatidylcholine Physiology medicine.medical_treatment Lung injury Dogs Pulmonary surfactant Ischemia Reference Values Physiology (medical) Hyperventilation medicine Animals Protein Isoforms Lung transplantation Enzyme Inhibitors Respiratory system Lung business.industry Respiratory disease Pulmonary Surfactants Cell Biology medicine.disease Lipids Phenylmethylsulfonyl Fluoride Transplantation medicine.anatomical_structure Acute Disease medicine.symptom business Lung Transplantation |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 282:L67-L74 |
| ISSN: | 1522-1504 1040-0605 |
| DOI: | 10.1152/ajplung.2002.282.1.l67 |
| Popis: | The effects of surfactant treatment on surfactant subtype conversion after lung injury were examined. Dogs were subjected to hyperventilation for 8 h with or without surfactant treatment. Lungs were stored for 17 h, and the right lung was transplanted and reperfused for 6 h. Conversion of large aggregate (LA) surfactant to small aggregates was investigated using in vitro surface area cycling. LA from transplanted lungs (Transplant-LA) from the nontreated group converted more rapidly than Transplant-LA from the treated group. Transplant-LA from both groups converted more rapidly than LA from normal lungs. Calculations based on [3H]dipalmitoylphosphatidylcholine in the administered surfactant [bovine lipid extract surfactant (BLES)] showed that the endogenous component of Transplant-LA converted more rapidly than the exogenous component. This indicates exogenous BLES did not equilibrate completely with endogenous surfactant. LA from hyperventilated, stored donor right lungs and from the recipients' native lungs from the nontreated group converted more rapidly than corresponding LA in the BLES-treated group. Similar relative conversions were observed with exogenous components from all lungs. Relative conversion of endogenous component from Transplant-LA was more rapid than that from LA from donor's stored right lung or from the recipient's native right lung. Low levels of phenylmethylsulfonyl fluoride inhibited conversion of Transplant-LA to a greater extent than normal LA. LA from all experimental groups had similar protein levels. These studies show acute lung injury, transplant, ischemia-reperfusion, and surfactant treatment have major effects on surfactant subtype integrity. |
| Databáze: | OpenAIRE |
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