Vitamin K1 and Vitamin K2 immunopharmacological effects on the peripheral lymphocytes of healthy subjects and dialysis patients, as estimated by the lymphocyte immunosuppressant sensitivity test

Autor:	Sachiko Tanaka, Junichi Kusano, Yoshiaki Fujii, Kentaro Sugiyama, Hiroto Matsuda, Masao Sekiyama, Shinya Suzuki, Yoshikazu Hara, Toshihiko Hirano, Eiki Ando
Rok vydání:	2018
Předmět:	0301 basic medicine Vitamin Adult Male Adolescent Lymphocyte Peripheral blood mononuclear cell T-Lymphocytes Regulatory Flow cytometry 03 medical and health sciences chemistry.chemical_compound Young Adult 0302 clinical medicine Renal Dialysis medicine Concanavalin A Humans Pharmacology (medical) IL-2 receptor Lymphocytes Aged Cell Proliferation Pharmacology Aged 80 and over medicine.diagnostic_test biology business.industry Vitamin K2 FOXP3 hemic and immune systems Vitamin K 2 Vitamin K 1 Middle Aged Kidney Transplantation 030104 developmental biology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Case-Control Studies Immunology biology.protein Leukocytes Mononuclear Cytokines Female Mitogens business Immunosuppressive Agents
Zdroj:	Journal of clinical pharmacy and therapeutics. 43(6)
ISSN:	1365-2710
Popis:	What is known and objective Renal transplant recipients receive immunosuppressive therapy to prevent acute rejection. We evaluated the immunopharmacological efficacy of vitamin K1 (VK1) and vitamin K2 (VK2) on T-cell mitogen-activated-peripheral lymphocytes of dialysis patients and healthy subjects. Methods The effects of VK1 and VK2 on the T-cell mitogen-stimulated proliferation of peripheral blood mononuclear cells (PBMCs) obtained from 12 healthy subjects and 12 dialysis patients were estimated. Seven cytokines produced from the activated PBMCs were measured by a BD Cytometric Beads Array kit. Regulatory T cells (Tregs) in PBMCs were analysed as CD4 + CD25 + FoxP3 + lymphocytes by flow cytometry. Results VK2 dose-dependently suppressed the concanavalin A-stimulated proliferation of PBMCs from healthy subjects and dialysis patients, whereas VK1 had no significant effect on the PBMC proliferation. VK1 and VK2 did not influence the production of most of the Th1/Th2/Th17 cytokines from the activated PBMCs of these subjects, although VK2 increased the IL-4 production from PBMCs of healthy subjects. The Treg percentages in the PBMCs of dialysis patients were markedly decreased compared to healthy PBMCs after the treatment with relatively low concentrations of VK2. What is new and conclusion The present data suggest that VK2 has immunosuppressive efficacy. VK2 may enhance the immunosuppressive efficacies of glucocorticoids while preventing osteoporosis caused by glucocorticoids.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a2861ab3ad54ff78d64a15c9ff814f25 https://pubmed.ncbi.nlm.nih.gov/30014604 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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