Aquaporins and Fetal Membranes From Diabetic Parturient Women: Expression Abnormalities and Regulation by Insulin
| Autor: | Philippe Deruelle, I. Fajardy, Damien Bouvier, Denis Gallot, Romain Lemarié, Geoffroy Marceau, Anne Vambergue, Bruno Pereira, Marion Rouzaire, C. Prat, Vincent Sapin, Loïc Blanchon |
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| Přispěvatelé: | Génétique, Reproduction et Développement (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), CHU Clermont-Ferrand, Service de Gynécologie - Obstétrique [Lille], Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Environnement périnatal et croissance - EA 4489 (EPS), Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Pôle Entrepreneuriat et Innovation - Rouen Business School, Rouen Business School, Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM), BLANCHON, LOIC |
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Extraembryonic Membranes Pregnancy in Diabetics Context (language use) Biology Aquaporins Biochemistry [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction Endocrinology Pregnancy Internal medicine Diabetes mellitus medicine Diabetes Mellitus Humans Insulin Amnion Fetus Aquaporin 3 [SDV.BDLR.RS] Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction Aquaporin 1 Biochemistry (medical) medicine.disease 3. Good health Gestational diabetes medicine.anatomical_structure Female Homeostasis |
| Zdroj: | Journal of Clinical Endocrinology and Metabolism Journal of Clinical Endocrinology and Metabolism, 2015, 100 (10), pp.E1270-E1279. ⟨10.1210/jc.2015-2057⟩ Journal of Clinical Endocrinology and Metabolism, Endocrine Society, 2015, 100 (10), pp.E1270-E1279. ⟨10.1210/jc.2015-2057⟩ Journal of Clinical Endocrinology and Metabolism, Vol. 100, No 10 (2015) pp. E1270-E1279 |
| ISSN: | 1945-7197 0021-972X |
| Popis: | Context: During pregnancy, aquaporins (AQPs) expressed in fetal membranes are essential for controlling the homeostasis of the amniotic volume, but their regulation by insulin was never explored in diabetic women. Objective: The aim of our study was to investigate the involvement of AQPs 1, 3, 8, and 9 expressed in fetal membranes in diabetic parturient women and the control of their expression by insulin. Design and Participants: From 129 fetal membranes in four populations (controls, type 1, type 2 [T2D], and gestational diabetes [GD]), we established an expression AQP profile. In a second step, the amnion was used to study the control of the expression and functions of AQPs 3 and 9 by insulin. Main Outcomes and Measures: The expression of transcripts and proteins of AQPs was studied by quantitative RT-PCR and ELISA. We analyzed the regulation by insulin of the expression of AQPs 3 and 9 in the amnion. A tritiated glycerol test enabled us to measure the impact of insulin on the functional characteristics. Using an inhibitor of phosphatidylinositol 3-kinase, we analyzed the insulin intracellular signaling pathway. Results: The expression of AQP3 protein was significantly weaker in groups T2D and GD. In nondiabetic fetal membranes, we showed for the amnion (but not for the chorion) a significant repression by insulin of the transcriptional expression of AQPs 3 and 9, which was blocked by a phosphatidylinositol 3-kinase inhibitor. Conclusion: In fetal membranes, the repression of AQP3 protein expression and functions observed in vivo is allowed by the hyperinsulinism described in pregnant women with T2D or GD. |
| Databáze: | OpenAIRE |
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