Association of ACE, VEGF and CCL2 gene polymorphisms with Henoch–Schonlein purpura and evaluating the possible interaction effects of these loci in HSP patients
| Autor: | Mandana Rafeey, Tahereh Mohammadian, Elahe Nabat, Mortaza Bonyadi |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A Henoch-Schonlein purpura Genotype IgA Vasculitis Medicine (miscellaneous) Disease Peptidyl-Dipeptidase A 030204 cardiovascular system & hematology Polymorphism Single Nucleotide General Biochemistry Genetics and Molecular Biology Pathogenesis 03 medical and health sciences 0302 clinical medicine Internal Medicine medicine Humans Genetic Predisposition to Disease Pharmacology (medical) Allele Child Gene Chemokine CCL2 Genetics (clinical) 030203 arthritis & rheumatology business.industry Case-control study Epistasis Genetic medicine.disease Molecular biology Case-Control Studies Reviews and References (medical) Immunology Female Gene polymorphism business |
| Zdroj: | Advances in Clinical and Experimental Medicine. 26:661-664 |
| ISSN: | 1899-5276 |
| DOI: | 10.17219/acem/62896 |
| Popis: | BACKGROUND Henoch-Schonlein purpura (HSP) is a multisystem, small vessel, leucocytoclastic vasculitis. It is predominantly a childhood vasculitis, rarely reported in adults. Studies have shown that several different genetic factors such as genes involved in inflammatory system and renin-angiotensin system (RAS) are important in the pathogenesis of Henoch-Schonlein purpura. OBJECTIVES The purpose of this study was to evaluate the independent effect of 3 gene polymorphisms including CCL2-2518 C/T, VEGF-634G/C and ACE(I/D) with HSP disease and their possible joint interactions in developing the disease. MATERIAL AND METHODS In this case-control study 47 HSP cases and 74 unrelated healthy controls were enrolled for evaluation. All individuals were genotyped for CCL2-2518C/T, VEGF-634G/C and ACE(I/D) gene polymorphisms. The possible association of these polymorphisms with susceptibility to develop HSP disease independently and in different joint combinations was evaluated. RESULTS The frequencies of TT genotype and T allele of CCL2-2518C/T gene polymorphism and CC genotype and C allele of VEGF-634G/C gene polymorphism were significantly high in HSP children (p-values = 0.005 and = 0.007 respectively). Interestingly, studying the joint interaction of these 2 genotypes (CC genotype of VEGF G-634C and TT genotype of CCL2 C-2518T) in this cohort showed a more significant effect in the development of the disease (p < 0.000, OR = 6.009). The frequency of TT genotype of CCL2 gene when combined with II genotype of ACE gene in HSP children was significantly higher (p < 0.000, OR = 4.213). CONCLUSIONS The results of this pilot study provide evidence of the possible gene-gene interaction effects of CCL2, VEGF and ACE genes in developing HSP disease. |
| Databáze: | OpenAIRE |
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