Integration of Single and Multicellular Wound Responses
| Autor: | Hoi Ying E Yu, Rhiannon R. Penkert, Andrew G. Clark, Ann L. Miller, Emily M. Vaughan, William M. Bement |
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| Jazyk: | angličtina |
| Předmět: |
rho GTP-Binding Proteins
Blastomeres Embryo Nonmammalian Cell Xenopus macromolecular substances Cell junction General Biochemistry Genetics and Molecular Biology Article Xenopus laevis medicine Animals Calcium Signaling Actin Calcium signaling Myosin Type II Wound Healing Agricultural and Biological Sciences(all) biology integumentary system Biochemistry Genetics and Molecular Biology(all) biology.organism_classification Epithelium Actins Cell biology Enzyme Activation Multicellular organism medicine.anatomical_structure Intercellular Junctions CELLBIO General Agricultural and Biological Sciences Wound healing |
| Zdroj: | Current Biology. (16):1389-1395 |
| ISSN: | 0960-9822 |
| DOI: | 10.1016/j.cub.2009.06.044 |
| Popis: | SummarySingle cells and multicellular tissues rapidly heal wounds. These processes are considered distinct, but one mode of healing—Rho GTPase-dependent formation and closure of a purse string of actin filaments (F-actin) and myosin-2 around wounds—occurs in single cells [1, 2] and in epithelia [3–10]. Here, we show that wounding of one cell in Xenopus embryos elicits Rho GTPase activation around the wound and at the nearest cell-cell junctions in the neighbor cells. F-actin and myosin-2 accumulate at the junctions and around the wound itself, and as the resultant actomyosin array closes over the wound site, junctional F-actin and myosin-2 become mechanically integrated with the actin and myosin-2 around the wound, forming a hybrid purse string. When cells are ablated rather than wounded, Rho GTPase activation and F-actin accumulation occur at cell-cell junctions surrounding the ablated cell, and the purse string closes the hole in the epithelium. Elevation of intracellular free calcium, an essential upstream signal for the single-cell wound response [2, 11], also occurs at the cell-cell contacts and in neighbor cells. Thus, the single and multicellular purse string wound responses represent points on a signaling and mechanical continuum that are integrated by cell-cell junctions. |
| Databáze: | OpenAIRE |
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