Cell motion predicts human epidermal stemness
| Autor: | Matome Imai, Ken Shiraishi, Koji Sayama, Yann Barrandon, Hiroshi Toki, Shigeki Higashiyama, Sota Tate, Daisuke Nanba, Natsuki Matsushita, Fujio Toki |
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| Předmět: |
Keratinocytes
Cellular differentiation Blotting Western Immunology Fluorescent Antibody Technique Cell Separation Integrin alpha6 Biology Real-Time Polymerase Chain Reaction Article Flow cytometry Cell therapy Cell Movement medicine Humans Immunology and Allergy Computer Simulation RNA Messenger Clonogenic assay Research Articles Cells Cultured Cell Proliferation medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Cell growth Stem Cells fungi food and beverages Cell Differentiation Cell Movement/physiology Epidermis/cytology Epidermis/metabolism Integrin alpha6/genetics Integrin alpha6/metabolism Keratinocytes/cytology Keratinocytes/metabolism RNA Messenger/genetics Stem Cells/cytology Stem Cells/metabolism Cell Biology Flow Cytometry Cell biology Transplantation medicine.anatomical_structure Epidermal Cells Epidermis Stem cell Keratinocyte |
| Zdroj: | Journal of Cell Biology, vol. 209, no. 2, pp. 305-315 The Journal of Cell Biology |
| Popis: | Keratinocyte stem cell colonies can be identified by analyzing cell motion, an emergent stem cell property. Image-based identification of cultured stem cells and noninvasive evaluation of their proliferative capacity advance cell therapy and stem cell research. Here we demonstrate that human keratinocyte stem cells can be identified in situ by analyzing cell motion during their cultivation. Modeling experiments suggested that the clonal type of cultured human clonogenic keratinocytes can be efficiently determined by analysis of early cell movement. Image analysis experiments demonstrated that keratinocyte stem cells indeed display a unique rotational movement that can be identified as early as the two-cell stage colony. We also demonstrate that α6 integrin is required for both rotational and collective cell motion. Our experiments provide, for the first time, strong evidence that cell motion and epidermal stemness are linked. We conclude that early identification of human keratinocyte stem cells by image analysis of cell movement is a valid parameter for quality control of cultured keratinocytes for transplantation. |
| Databáze: | OpenAIRE |
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