Epi-laser in situ keratomileusis: Comparative evaluation of epithelial separation with 3 microkeratomes
| Autor: | Wolfgang A. Herrmann, Philipp Prahs, Horst Helbig, Chris P. Lohmann, Karsten Hufendiek, Karin Kobuch, Jost Hillenkamp |
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| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
Collagen Type VII genetic structures Cell Survival Swine medicine.medical_treatment Keratomileusis Laser In Situ Keratomileusis Surgical Flaps Cornea Immunoenzyme Techniques chemistry.chemical_compound Ophthalmology Microkeratome medicine Animals Humans Coloring Agents Basement membrane Integrin beta4 Epithelium Corneal Histology Trypan Blue Anatomy Lamina lucida eye diseases Sensory Systems Epithelium medicine.anatomical_structure chemistry Lasers Excimer Surgery Trypan blue Lamina densa sense organs Cell Adhesion Molecules Biomarkers |
| Zdroj: | Journal of Cataract and Refractive Surgery. 34:1761-1766 |
| ISSN: | 0886-3350 |
| DOI: | 10.1016/j.jcrs.2008.06.028 |
| Popis: | Purpose To evaluate the cleavage plane, corneal cytoarchitecture, and cell vitality of separated corneal epithelial sheets created with 3 commonly used microkeratomes. Setting Laboratories of the Regensburg University Medical Center, Regensburg, Germany. Methods Mechanical separation of the epithelial layer in 10 porcine eyes and 2 human eyes was performed with 3 different microkeratomes (Amadeus II, Zyoptix XP, Epivision). Five of 10 porcine corneas and the 2 human corneas treated with each microkeratome were processed for histology, electron microscopy, and immunohistochemistry. In 5 of 10 porcine corneas, the corneal epithelial sheets were separated from the globe and cell vitality was assessed with the trypan blue dye vitality test. Results A reproducible epithelial separation with a smooth surface was achieved in all eyes. The cleavage plane was located between the lamina lucida and the lamina densa. Damage to epithelial cells was mainly limited to the cut margins. Conclusions Mechanical separation of the epithelial sheet in epithelial laser in situ keratomileusis (epi-LASIK) was safe and reproducible with all evaluated microkeratomes. Immunohistochemistry and electron microscopy showed the cleavage plane in epi-LASIK was between the basal epithelium and the basement membrane at the level of the lamina lucida. |
| Databáze: | OpenAIRE |
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