Alkylphenolic contaminants in the diet: Sparus aurata juveniles hepatic response
| Autor: | D.G. Mita, Alberta Mandich, A. Scorolli, Ilaria Traversi, Giorgia Gioacchini, Oliana Carnevali |
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| Rok vydání: | 2014 |
| Předmět: |
Liver morphology
medicine.medical_specialty Food Contamination Biology Real-Time Polymerase Chain Reaction Vitellogenins chemistry.chemical_compound Endocrinology Phenols Biotransformation Adverse health effect Internal medicine medicine Animals Endocrine system Gonadal Steroid Hormones Phenol Body Weight Egg Proteins Organ Size Hepatic tissue Contamination Lipid Metabolism Sea Bream Diet Nonylphenol Liver chemistry Animal Science and Zoology Biomarkers Hormone |
| Zdroj: | General and Comparative Endocrinology. 205:185-196 |
| ISSN: | 0016-6480 |
| DOI: | 10.1016/j.ygcen.2014.06.015 |
| Popis: | A wide range of endocrine disrupter chemicals can mimic steroid hormones causing adverse health effects. Nonylphenol (NP) and t-octhylphenol (t-OP) are man-made alkylphenolic environmental contaminants possessing controversial endocrine disruption properties. This study has investigated the effects of NP and t-OP enriched diets on hepatic tissue and biotransformation activities in the liver. To this aim, sea bream juveniles were fed with commercial diet enriched with three different doses of NP (NP1: 5mg/kg bw, NP2: 50mg/kg bw and NP3: 100mg/kg bw) or t-OP (t-OP1: 5mg/kg bw, t-OP2: 50mg/kg bw and t-OP3: 100mg/kg bw) for 21 days. A significant increase of the hepatosomatic index was observed in NP1 and t-OP1. Alteration of liver morphology was observed in both NP and t-OP exposed juveniles although the most altered endpoints were observed in t-OP2 with 100% of tissue degeneration. Ethoxyresorufin-O-deethylase activity was significantly inhibited by NP and t-OP (p0.05), while catalase activity was significantly induced, at both doses. A different pattern of protein expression of different isoforms of both vitellogenin and zona radiata protein was evidenced within the treatments. In addition, a significant increase in the abundance of the stress induced heat shock protein 70 gene in the liver of t-OP2 fish and a significant increase in the abundance of the estrogen induced cathepsin D gene in the liver of NP1 and t-OP2 fish, were observed. Finally, estradiol-17β (E2) and testosterone (T) plasma levels and E2/T showed significantly different patterns in NP and t-OP exposed against control fish. |
| Databáze: | OpenAIRE |
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