The application of lentiviral vectors for the establishment of TGFβ2-induced ocular hypertension in C57BL/6J mice

Autor:	Michael Peng, Tyler J. Margetts, Naga Pradeep Rayana, Chenna Kesavulu Sugali, Jiannong Dai, Weiming Mao
Rok vydání:	2022
Předmět:	Male Sensory Systems Adenoviridae Mice Inbred C57BL Mice Transforming Growth Factor beta2 Cellular and Molecular Neuroscience Ophthalmology Trabecular Meshwork Cytomegalovirus Infections Animals Female Ocular Hypertension Glaucoma Open-Angle Intraocular Pressure
Zdroj:	Experimental Eye Research. 221:109137
ISSN:	0014-4835
DOI:	10.1016/j.exer.2022.109137
Popis:	Elevated levels of TGFβ2 in the aqueous humor is associated with the pathological changes in the trabecular meshwork (TM). These changes lead to ocular hypertension (OHT), the most important risk factor for the development and progression of primary open angle glaucoma (POAG), a leading cause of blindness worldwide. Therefore, TGFβ2 is frequently used to develop OHT models including in perfusion cultured eyes and in mouse eyes. Adenovirus-mediated overexpression of human mutant TGFβ2 has demonstrated great success in increasing intraocular pressure (IOP) in mouse eyes. However, adenoviruses have limited capacity for a foreign gene, induce transient expression, and may cause ocular inflammation. Here, we explored the potential of using lentiviral vectors carrying the mutant human TGFβ2
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a275dd79814f1c1dde3e50013c3526f4 https://doi.org/10.1016/j.exer.2022.109137 Zobrazit plný text záznamu Full Text from ScienceDirect