Genome-wide identification of calcium-dependent protein kinases in soybean and analyses of their transcriptional responses to insect herbivory and drought stress
| Autor: | Jianqiang Wu, Christian Hettenhausen, Jinfeng Qi, Huifu Zhuang, Yanbiao He, Guiling Sun, Ting Sun |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Transcription Genetic Response Elements 01 natural sciences Chromosomes Plant Article 03 medical and health sciences chemistry.chemical_compound Plant Growth Regulators Gene Expression Regulation Plant Stress Physiological Botany Gene family Herbivory Soybean aphid Promoter Regions Genetic Gene Abscisic acid Phylogeny Regulation of gene expression Genetics Multidisciplinary biology Abiotic stress Gene Expression Profiling Jasmonic acid fungi Chromosome Mapping food and beverages biology.organism_classification Droughts Gene expression profiling 030104 developmental biology chemistry Soybeans Transcriptome Protein Kinases Genome-Wide Association Study 010606 plant biology & botany |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep18973 |
| Popis: | Calcium-dependent protein kinases (CDPKs) are plant-specific calcium sensors that play important roles in various aspects of plant physiology. Here, we investigated phylogenic relationships, chromosomal locations, gene structures and tissue-specific, herbivory- and drought-induced expression profiles of soybean (Glycine max) GmCDPKs. Fifty GmCDPK genes were identified, which phylogenetically grouped into 4 distinct clusters and distributed across 13 sub-clusters. Individual classes of GmCDPKs harbor highly conserved mRNA splicing sites and their exon numbers and lengths were consistent with the phylogenetic relationships, suggesting that at least 13 ancestral CDPK genes had emerged before the split of monocots and eudicots. Gene expression analysis indicated that several GmCDPKs were tissue-specific expressed. GmCDPKs’ transcript levels changed after wounding, exhibited specific expression patterns after simulated Spodoptera exigua feeding or soybean aphid (Aphis glycines) herbivory and were largely independent of the phytohormones jasmonic acid and salicylic acid. The most pronounced transcriptional responses were detected after drought and abscisic acid treatments with more than half of all GmCDPKs being upregulated, suggesting their important roles during abiotic stress responses in soybean. Our data provide an important foundation for further functional dissection of GmCDPKs, especially in the context of soybean-insect interactions and drought stress adaptation. |
| Databáze: | OpenAIRE |
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