Comparison of the therapeutic effects of adipose‑derived and bone marrow mesenchymal stem cells on erectile dysfunction in diabetic rats
| Autor: | Mingzhu Wang, Jingjing Li, Jianbin Zhu, Hongwei Li, Yi Wang, Qiu Zhuolin, Mingyu Xu, Sansan Chen, Xinglu Chen, Liu Jun, Yang Xiaorong, Huiying Chen, She Miaoqin, Huang Yanting, Xiangsheng Cai |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Nitric Oxide Synthase Type III erectile dysfunction H&E stain Adipose tissue Intracavernous injection bone marrow stem cells Mesenchymal Stem Cell Transplantation Diabetes Complications 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Genetics medicine Animals Sirius Red diabetes business.industry Penile Erection Mesenchymal stem cell Mesenchymal Stem Cells General Medicine Articles Staining Rats Transplantation 030104 developmental biology medicine.anatomical_structure chemistry Adipose Tissue 030220 oncology & carcinogenesis Bone marrow Collagen adipose-derived stem cells business Biomarkers |
| Zdroj: | International Journal of Molecular Medicine |
| ISSN: | 1791-244X |
| Popis: | The aim of the present study was to compare the effects of adipose‑derived mesenchymal stem cell (ADSC) and bone marrow mesenchymal stem cell (BMSC) transplantation into the corpora cavernosa of diabetic rats with erectile function. ADSCs and BMSCs were isolated and identified by flow cytometry. Rats with streptozocin‑induced diabetes were screened using apomorphine to obtain a rat model of diabetic erectile dysfunction, followed by transplantation of ADSCs and BMSCs into the corpora cavernosa. Two weeks later, the rats were again injected with apomorphine, the intracavernous pressure (ICP) and mean arterial pressure (MAP) of the penile tissue were measured, and the corpus cavernosum tissues were harvested. Angiogenic endothelial nitric oxide synthase (eNOS) expression was detected by western blotting and immunofluorescence analysis. The blood vessels in the corpus cavernosum were observed following hematoxylin and eosin (H&E) staining, and the expression of collagen was detected by Sirius Red staining. The cellular ultrastructure was examined by transmission electron microscopy. Intracavernous injection of ADSCs significantly increased ICP and ICP/MAP. Western blotting and immunofluorescence results revealed that ADSC treatment improved the expression of eNOS in the penile tissue of diabetic rats. The H&E staining results demonstrated that ADSC treatment promoted revascularization of the corpus cavernosum, and the results of Sirius Red staining revealed that ADSC treatment reduced penile collagen in diabetic rats. Transmission electron microscopy examination revealed that the ultrastructure of the tissues in the ADSC‑treated group was more complete compared with that in the untreated diabetic model group. In conclusion, ADSCs were found to be more effective compared with BMSCs in treating diabetes‑related erectile dysfunction. |
| Databáze: | OpenAIRE |
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