Distinct gene mutation profiles among luminal-type and basal-type breast cancer cell lines

Autor: John W.M. Martens, Mieke Schutte, Ellen C. Zwarthoff, Pim J. French, Amina F A S Teunisse, Hans Clevers, Marieke J. Rozendaal, Stephen P. Ethier, Aart G. Jochemsen, Peter J. van der Spek, Suzanne Lam, Muhammad Riaz, John A. Foekens, Marijke Wasielewski, Winand N.M. Dinjens, Michael A. den Bakker, Bertie de Leeuw, Timo L.M. ten Hagen, Antoinette Hollestelle, Ser Sue Ng, Jan G. M. Klijn, Fons Elstrodt, Daphne G. Koopman, Marcel Smid, Jord H. A. Nagel, Justine K. Peeters
Přispěvatelé: Hubrecht Institute for Developmental Biology and Stem Cell Research, Department of Medical Oncology, Josephine Nefkens Institute, Erasmus University Medical Center [Rotterdam] (Erasmus MC), Cancer Genomics Centre, Department of Molecular Cell Biology, Leiden University Medical Center (LUMC), Department of Neurology, Josephine Nefkens Institute, Department of Bioinformatics, Laboratory Experimental Surgical Oncology, Section Surgical Oncology, Department of Surgery, Daniel den Hoed Cancer Center, Department of Pathology, Josephine Nefkens Institute, Barbara Ann Karmanos Cancer Institute, Wayne State University School of Medicine, Medical Oncology, Neurology, Hematology, Erasmus MC other, Surgery, Pathology
Rok vydání: 2009
Předmět:
Cancer Research
Breast cancer subtypes Histological classification Intrinsic subtypes Molecular classification Mutation analysis e-cadherin colorectal cancers pathway mutations tumor-suppressor m14 melanoma carcinoma expression subtypes p53 mutants
Breast cancer subtypes
DNA Mutational Analysis
Gene Expression
Breast Neoplasms
Biology
Gene mutation
medicine.disease_cause
03 medical and health sciences
0302 clinical medicine
Cyclin D1
Breast cancer
p14arf
SDG 3 - Good Health and Well-being
Cell Line
Tumor
medicine
Biomarkers
Tumor
Humans
skin and connective tissue diseases
Gene
Histological classification
030304 developmental biology
Genetics
0303 health sciences
Mutation
Gene Expression Profiling
medicine.disease
3. Good health
Gene expression profiling
Mutation analysis
Oncology
Intrinsic subtypes
Molecular classification
030220 oncology & carcinogenesis
Cancer research
Female
Carcinogenesis
Zdroj: Breast Cancer Research and Treatment, 121(1), 53-64
Breast Cancer Research and Treatment. Springer New York
Breast Cancer Research and Treatment
Breast Cancer Research and Treatment, Springer Verlag, 2009, 121 (1), pp.53-64. ⟨10.1007/s10549-009-0460-8⟩
Breast Cancer Research and Treatment, 121(1), 53-64. Springer New York
ISSN: 1573-7217
0167-6806
DOI: 10.1007/s10549-009-0460-8⟩
Popis: Breast cancer has for long been recognized as a highly diverse tumor group, but the underlying genetic basis has been elusive. Here, we report an extensive molecular characterization of a collection of 41 human breast cancer cell lines. Protein and gene expression analyses indicated that the collection of breast cancer cell lines has retained most, if not all, molecular characteristics that are typical for clinical breast cancers. Gene mutation analyses identified 146 oncogenic mutations among 27 well-known cancer genes, amounting to an average of 3.6 mutations per cell line. Mutations in genes from the p53, RB and PI3K tumor suppressor pathways were widespread among all breast cancer cell lines. Most important, we have identified two gene mutation profiles that are specifically associated with luminal-type and basal-type breast cancer cell lines. The luminal mutation profile involved E-cadherin and MAP2K4 gene mutations and amplifications of Cyclin D1, ERBB2 and HDM2, whereas the basal mutation profile involved BRCA1, RB1, RAS and BRAF gene mutations and deletions of p16 and p14ARF. These subtype-specific gene mutation profiles constitute a genetic basis for the heterogeneity observed among human breast cancers, providing clues for their underlying biology and providing guidance for targeted pharmacogenetic intervention in breast cancer patients.
Databáze: OpenAIRE
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