Segregation of lens and olfactory precursors from a common territory: cell sorting and reciprocity of Dlx5 and Pax6 expression
| Autor: | Sujata Bhattacharyya, Marianne Bronner-Fraser, Andrea Streit, Andrew P. Bailey |
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| Rok vydání: | 2004 |
| Předmět: |
Fate map
Distal-less Dlx PAX6 Transcription Factor Video Recording Ectoderm Nasal epithelium Chick Embryo Biology Chick Eye Olfactory Mucosa Fate mapping Cell Movement Lens Crystalline medicine Morphogenesis Animals Paired Box Transcription Factors Lens placode Cell Lineage Placode Olfactory Region Eye Proteins Eyeless Molecular Biology In Situ Hybridization Homeodomain Proteins Sensory organ Gene Expression Regulation Developmental Anatomy Cell Biology Immunohistochemistry Pax6 Cell biology Repressor Proteins medicine.anatomical_structure Electroporation Neural Crest Lens (anatomy) PAX6 Neural plate Olfactory epithelium Developmental Biology |
| Zdroj: | Developmental Biology. 271(2):403-414 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2004.04.010 |
| Popis: | Cranial placodes are focal regions of columnar epithelium next to the neural tube that contribute to sensory ganglia and organs in the vertebrate head, including the olfactory epithelium and the crystalline lens of the eye. Using focal dye labelling within the presumptive placode domain, we show that lens and nasal precursors arise from a common territory surrounding the anterior neural plate. They then segregate over time and converge to their final positions in discrete placodes by apparently directed movements. Since these events closely parallel the separation of eye and antennal primordia (containing olfactory sensory cells) from a common imaginal disc in Drosophila, we investigated whether the vertebrate homologues of Distalless (Dll) and Eyeless (Ey), which determine antennal and eye identity in the fly, play a role in segregation of lens and nasal precursors in the chick. Dlx5 and Pax6 are initially co-expressed by future lens and olfactory cells. As soon as presumptive lens cells acquire columnar morphology all Dlx family members are down-regulated in the placode, while Pax6 is lost in the olfactory region. Lens precursor cells that express ectopic Dlx5 never acquire lens-specific gene expression and are excluded from the lens placode to cluster in the head ectoderm. These results suggest that the loss of Dlx5 is required for cells to adopt a lens fate and that the balance of Pax6 and Dlx expression regulates cell sorting into appropriate placodal domains. |
| Databáze: | OpenAIRE |
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